| LDR | | 02812nam u200481 4500 |
| 001 | | 000000418133 |
| 005 | | 20190215162616 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780355870220 |
| 035 | |
▼a (MiAaPQ)AAI10747458 |
| 035 | |
▼a (MiAaPQ)duke:14437 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 576 |
| 100 | 1 |
▼a Esher, Shannon Kimberley. |
| 245 | 10 |
▼a Mechanisms of Protein Localization in Cryptococcus neoformans Mediate Virulence and Immune Recognition. |
| 260 | |
▼a [S.l.]:
▼b Duke University.,
▼c 2018. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2018. |
| 300 | |
▼a 246 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B. |
| 500 | |
▼a Adviser: J. Andrew Alspaugh. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Duke University, 2018. |
| 506 | |
▼a This item is not available from ProQuest Dissertations & Theses. |
| 520 | |
▼a Cryptococcus neoformans is an opportunistic fungal pathogen that causes significant disease and death in immunocompromised populations, in particular among those with advanced HIV infection. This fungus is found ubiquitously in the environment a |
| 520 | |
▼a Fungal growth and morphogenesis, as well as thermotolerance and virulence are controlled by conserved Ras-like GTPases. These proteins require proper localization for full function and are directed to cellular membranes through the posttranslati |
| 520 | |
▼a C. neoformans dramatically alters its cell wall upon entering the host in order to facilitate immune avoidance. Using the tools of forward genetics, we identified a novel cell wall regulatory protein, Mar1. We have demonstrated that this protein |
| 520 | |
▼a To further understand the impact of the Mar1 protein on the host-pathogen interaction, we used in vivo mouse models to characterize the pathogenesis and immune response to this strain. Using histopathology and light microscopy, we have shown tha |
| 520 | |
▼a Lastly, using bioinformatics tools and batch sampling, we developed a novel computational tool to more efficiently analyze mutants of interest generated by forward genetic screens. We demonstrate the efficacy of this tool through proof of princi |
| 590 | |
▼a School code: 0066. |
| 650 | 4 |
▼a Microbiology. |
| 650 | 4 |
▼a Genetics. |
| 650 | 4 |
▼a Molecular biology. |
| 690 | |
▼a 0410 |
| 690 | |
▼a 0369 |
| 690 | |
▼a 0307 |
| 710 | 20 |
▼a Duke University.
▼b Molecular Genetics and Microbiology. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-09B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0066 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14996929
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a ***1012033 |