| LDR | | 02355nam u200409 4500 |
| 001 | | 000000422593 |
| 005 | | 20190215170242 |
| 008 | | 181129s2017 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438309654 |
| 035 | |
▼a (MiAaPQ)AAI10970723 |
| 035 | |
▼a (MiAaPQ)OhioLINK:osu1500561466281889 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 540 |
| 100 | 1 |
▼a DeSantis, Christopher Amedeo. |
| 245 | 10 |
▼a Rna Kissing-Loop Interactions Supported By Bifacial Peptide Nucleic Acid. |
| 260 | |
▼a [S.l.]:
▼b The Ohio State University.,
▼c 2017. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2017. |
| 300 | |
▼a 291 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 80-01(E), Section: B. |
| 500 | |
▼a Adviser: Dennis Bong,Dr. |
| 502 | 1 |
▼a Thesis (Ph.D.)--The Ohio State University, 2017. |
| 520 | |
▼a The dissertation to follow is an account of research efforts to utilize bifacial peptide nucleic acids (bPNA) triplexes to support kissing-loop complexes. A kissing-loop complex is composed of two nucleic acid stem-loops hybridized via Watson-Cr |
| 520 | |
▼a In the first part if this work, kissing-loop and Rop complex formation was analyzed in titration binding assays and native gel electrophoresis. tRNA duplex-triplex kissing complexes show a 5-fold decrease in affinity while omission of bPNA decre |
| 520 | |
▼a In the latter part of this work, fluorescently-labeled bPNA were used to monitor kissing-loop formation in vitro. Triplex-triplex kissing-loop formation was monitored by FRET using Cy3- and Cy5-labeled bPNA triplexes. Competitive displacement of |
| 520 | |
▼a In summary, we have shown that hairpins mutated with a bPNA triplex in the stem region are able to form kissing-loop complexes. Furthermore, proteins which interact with kissing-loops are still able to bind complexes with the triplex mutation. U |
| 590 | |
▼a School code: 0168. |
| 650 | 4 |
▼a Chemistry. |
| 690 | |
▼a 0485 |
| 710 | 20 |
▼a The Ohio State University.
▼b Chemistry. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 80-01B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0168 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2017 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15001332
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a ***1012033 |