| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000431836 |
| 005 | | 20200224105707 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781088302958 |
| 035 | |
▼a (MiAaPQ)AAI13899130 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 617 |
| 100 | 1 |
▼a Bamashmous, Shatha. |
| 245 | 10 |
▼a Investigation of Chemokine and Microbiome Profiles in Gingival Health and Disease in Humans. |
| 260 | |
▼a [S.l.]:
▼b University of Washington.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 168 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-04, Section: B. |
| 500 | |
▼a Advisor: Darveau, Richard P. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Washington, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 506 | |
▼a This item must not be added to any third party search indexes. |
| 520 | |
▼a Periodontal health is maintained by various host immune mechanisms. Neutrophils are a crucial component of health representing the first line of defense against microbial challenge. The migration and activation of these key effector cells in the gingiva are orchestrated by complex networks of host mediators called chemokines and cytokines. Recently, it has been shown that bacteria have an influential role in the immunomodulation of host mediators and hence host immune response. Indeed, dysregulation of the host immune response, as well as a dysbiosis of the oral microbial community, has been attributed to the pathogenesis of periodontal disease. However, the few investigations into the interaction between the subgingival microbial community and the host immune response have provided limited information to understand this relationship. Therefore, a full assessment of the host-bacterial interaction in health and disease is necessary to further our understandings of the etiology of periodontal disease and identify novel biomarkers of this disease.This thesis investigates the interaction of subgingival microbiome and the host immune response for both health and disease with disease defined as induced gingivitis. A comprehensive evaluation of forty major chemokines and inflammatory mediators in the gingival crevicular fluid (GCF) was achieved by multiplex immunoassay. In parallel, 16S rRNA next-generation sequencing was performed to characterize microbial community composition and structure. Lastly, neutrophil infiltration was assessed by myeloperoxidase (MPO) detection in the GCF to correlate neutrophil recruitment with chemokine expression patterns.The cross-sectional study in healthy adolescents showed inter-individual variability in the chemokine expression patterns and the subgingival microbial profile. Two different chemokine expression patterns were observed among study participants |
| 590 | |
▼a School code: 0250. |
| 650 | 4 |
▼a Dentistry. |
| 650 | 4 |
▼a Microbiology. |
| 650 | 4 |
▼a Immunology. |
| 650 | 4 |
▼a Dental research. |
| 690 | |
▼a 0567 |
| 690 | |
▼a 0410 |
| 690 | |
▼a 0982 |
| 710 | 20 |
▼a University of Washington.
▼b Oral Biology. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-04B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0250 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15492023
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |