| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000431919 |
| 005 | | 20200224110825 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781085796071 |
| 035 | |
▼a (MiAaPQ)AAI13887091 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 581 |
| 100 | 1 |
▼a Carter, Jason William. |
| 245 | 10 |
▼a Fusarium circinatum, the Cause of Pitch Canker in Pines, and Its Interactions with Pine and Grass Hosts. |
| 260 | |
▼a [S.l.]:
▼b University of California, Davis.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 81 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-04, Section: B. |
| 500 | |
▼a Advisor: Gordon, Thomas R. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of California, Davis, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 520 | |
▼a Chapter 1 of this dissertation describes assays of 2-benzoxazolinone (BOA) tolerance in a genetically diverse population of Fusarium circinatum Nirenberg & O'Donnell, the F1 progeny of mating between two wild-type California isolates. Tolerance was assayed in vitro by observing isolate growth on a PDA+BOA0.5mg/ml medium. Fusarium circinatum exhibited a range of BOA tolerances in a diverse population of isolates. BOA tolerance may be calculated as growth on PDA+BOA0.5mg/ml as % of growth on PDA at 7DPI. We conducted a series of matings in which parents were selected for either high or low BOA tolerance. Significant shifts in BOA tolerance were observed in F2 and F3 progeny compared to the F1 population.Chapter 2 describes the capacity for eight F. circinatum isolates with differential BOA tolerance, four with high BOA tolerance and four with low BOA tolerance, to infect Zea mays L. and induce lesions on Pinus radiata D. Don. Based on lesion lengths, isolates differed significantly in their virulence to P. radiata. High BOA tolerating isolates produced significantly longer lesions on P. radiata than low BOA tolerating isolates. Zea mays was inoculated with F. circinatum using two methods: soaking seeds in spore suspensions, and growing Z. mays in sand infested with F. circinatum. Seed-soak experiments harvested and cultured both above and below ground Z. mays tissue, which showed no significant differences between F. circinatum isolates in their capacity to infect grasses. Fusarium circinatum BOA tolerance groups also did not significantly differ in their grass infection capacity. All F. circinatum isolates were capable of infecting both root and shoot tissues. With plants cultivated in infested sand, fungal infection was measured with a quantitative polymerase chain reaction (qPCR) approach. Using DNA extracts from both belowground mesocotyl and aboveground shoot tissue, fungal DNA was measured relative to plant DNA. qPCR data showed no significant difference between isolates or BOA tolerance groups for fungal presence in Z. mays tissue.Chapter 3 describes a study of colonization of the native California grass, Bromus carinatus Hook. & Arn., by Fusarium circinatum. At Point Reyes National Seashore in California, Fusarium circinatum, the causal agent of pitch canker in pines, was isolated from Pinus muricata D. Don, the California native grass, Bromus carinatus, and the introduced grass, Holcus lanatus L. All grass plants from which F. circinatum was isolated were symptomless. Pathogenicity of grass isolates was confirmed by inoculation of Pinus radiata trees, which developed symptoms similar to trees inoculated with a pine isolate of F. circinatum. Isolates from grasses were somatically compatible with isolates recovered from symptomatic pines. Bromus carinatus grown in a growth chamber was inoculated with a green fluorescent protein expressing strain of F. circinatum. Segments of inoculated leaves were incubated in moist chambers, and after one to two days, sporulating hyphae were observed growing from leaf tissue. Spores of F. circinatum removed from B. carinatus leaves were confirmed to be fluorescent when illuminated with ultraviolet light. These results raise the possibility that B. carinatus cryptically infected by F. circinatum may be a source of propagules capable of infecting pines. |
| 590 | |
▼a School code: 0029. |
| 650 | 4 |
▼a Plant pathology. |
| 690 | |
▼a 0480 |
| 710 | 20 |
▼a University of California, Davis.
▼b Plant Biology. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-04B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0029 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15491554
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |