| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000432494 |
| 005 | | 20200224131346 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781088325438 |
| 035 | |
▼a (MiAaPQ)AAI13898397 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 578 |
| 100 | 1 |
▼a Gagnon, Lauren Ann. |
| 245 | 10 |
▼a Advancements in Single Molecule Localization Microscopy for Improved Multichannel, 3-Dimensional, and Structurally-Dense Imaging. |
| 260 | |
▼a [S.l.]:
▼b University of Washington.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 112 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-04, Section: B. |
| 500 | |
▼a Includes supplementary digital materials. |
| 500 | |
▼a Advisor: Vaughan, Joshua C. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Washington, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 506 | |
▼a This item must not be added to any third party search indexes. |
| 520 | |
▼a Super-resolution fluorescence microscopy now enables cellular detail to be probed at the nanometer scale using far-field optics with widely available instrumentation. However, single molecule localization microscopy (SMLM) techniques that rely on the stochastic on-off switching of individual fluorophores to achieve higher resolution face significant drawbacks when trying to achieve multicolor, 3-D, and structurally-dense imaging. This is due to limitations in the photoswitchable fluorophores currently available. Here, I provide a detailed discussion of photoswitchable fluorophores for SMLM and the challenges they create. I also present a new method, prSTORM, which is capable of using a single fluorophore to image all channels and achieve 3-D imaging at extended depths through the use of DNA barcodes. Additionally, I propose how this methodology can be extended to high structural density imaging without compromising resolution. |
| 590 | |
▼a School code: 0250. |
| 650 | 4 |
▼a Chemistry. |
| 650 | 4 |
▼a Microscopy. |
| 690 | |
▼a 0485 |
| 710 | 20 |
▼a University of Washington.
▼b Chemistry. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-04B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0250 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15491937
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |