| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000432529 |
| 005 | | 20200224131912 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781085758536 |
| 035 | |
▼a (MiAaPQ)AAI13902471 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 576 |
| 100 | 1 |
▼a Bone, Mary Ashley. |
| 245 | 10 |
▼a Understanding the Regulatory Dynamic of the Two-component Systems, PlrSR and BvgAS, During Bordetella Colonization of the Mammalian Respiratory Tract. |
| 260 | |
▼a [S.l.]:
▼b The University of North Carolina at Chapel Hill.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 137 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-02, Section: B. |
| 500 | |
▼a Advisor: Cotter, Peggy A. |
| 502 | 1 |
▼a Thesis (Ph.D.)--The University of North Carolina at Chapel Hill, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 520 | |
▼a Pertussis is a severe respiratory disease caused by the bacterium Bordetella pertussis. Despite high vaccination coverage, the number of pertussis cases has rebounded in recent years. To combat this disease, more efficacious vaccines and improved knowledge of Bordetella virulence is critical. Bacteria often use two-component systems (TCSs) to coordinate essential cellular processes in response to the diverse environments they encounter. In B. pertussis and the closely related subspecies B. bronchiseptica, the TCS, BvgAS, controls the expression of almost all known virulence factor-encoding genes and is considered the main virulence regulatory system. Recently, another TCS, PlrSR, was identified that is also required for Bordetella infection within the lower respiratory tract (LRT). However, why PlrSR is important for colonization is unknown. Using engineered strains of B. bronchiseptica and genetic reporters, we demonstrated that PlrS is required for the maintenance of BvgAS activity in the lungs of mice, indicating that PlrSR, along with BvgAS, coordinates virulence specifically in the LRT. Moreover, our data indicate that PlrSR may regulate genes that are required for persistence in the LRT independent of BvgAS. Importantly, these genes and their corresponding proteins may serve as new vaccine components or therapeutic targets. We have also shown that the Per-Arnt-Sim (PAS) domain of BvgS is required for BvgS inactivation in the absence of PlrS, indicating that the PAS domain is important for the PlrSR-BvgAS connection and functions as an independent signaling domain. Based on published data and our findings, we hypothesize that PlrSR controls the expression of high-affinity cytochrome oxidases (HACOs) that are required for bacterial respiration and maintenance of BvgAS activity in the LRT. We have demonstrated that two of four HACOs contribute to LRT colonization. However, BvgAS activity is unaffected by the loss of these HACOs and it is unknown if PlrSR regulates the expression of any HACO encoding genes. Together, this work provides a more detailed understanding of the coordinated regulation imposed by PlrSR and BvgAS to promote Bordetella survival within the mammalian host. |
| 590 | |
▼a School code: 0153. |
| 650 | 4 |
▼a Microbiology. |
| 690 | |
▼a 0410 |
| 710 | 20 |
▼a The University of North Carolina at Chapel Hill.
▼b Microbiology and Immunology. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-02B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0153 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15492369
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |