| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000432641 |
| 005 | | 20200224133218 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781392259320 |
| 035 | |
▼a (MiAaPQ)AAI13895964 |
| 035 | |
▼a (MiAaPQ)indiana:15806 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 581 |
| 100 | 1 |
▼a Helm, Matthew David. |
| 245 | 10 |
▼a Engineering Novel Disease Resistance Traits in Crop Plants. |
| 260 | |
▼a [S.l.]:
▼b Indiana University.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 272 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 80-12, Section: B. |
| 500 | |
▼a Publisher info.: Dissertation/Thesis. |
| 500 | |
▼a Advisor: Innes, Roger W. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Indiana University, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 520 | |
▼a Improving crop yields in an environmentally sustainable manner requires, in part, the development of disease resistant crop plants. We have previously shown that the effector protease AvrPphB from Pseudomonas syringae is recognized in Arabidopsis via an indirect mechanism in which the disease resistance protein RPS5 is activated by sensing proteolytic cleavage of the Arabidopsis PBS1 protein. We have also shown that we can expand the recognition specificity of RPS5 by changing the protease cleavage site within PBS1 such that it is cleaved by other pathogen proteases, thereby conferring resistance against multiple pathogens. I refer to these modified PBS1 proteins as "PBS1 decoys". In this dissertation, I extend upon our previous work to show that I can engineer a novel disease resistance trait in soybean using endogenous soybean PBS1 genes. Specifically, I show that a soybean PBS1 decoy protein modified to contain a cleavage site sequence for the Soybean mosaic virus (SMV) NIa protease triggers cell death in soybean protoplasts when cleaved by this protease and this resistance response inhibits systemic spread of SMV in soybean. Second, I demonstrate that barley recognizes AvrPphB protease activity and contains AvrPphB-cleavable PBS1 proteins, similar to Arabidopsis. I show that the AvrPphB response maps to a single locus containing a resistance gene, which I termed AvrPphB Response1 (Pbr1), and confirmed that PBR1 mediates recognition of AvrPphB protease activity and associates with barley PBS1 proteins in planta. Phylogenetic analyses showed that Pr1 is not orthologous to RPS5 hence the ability to recognize AvrPphB protease activity has evolved independently in monocots and dicots. Lastly, I show that wheat recognizes AvrPphB and contains AvrPphB-cleavable PBS1 proteins. Further, wheat encodes two homoeologous Pbr1 genes located in a position syntenic with barley Pbr1 suggesting wheat also contains a conserved AvrPphB recognition system. Through my dissertation research, I have provided evidence showing that the molecular mechanism used to detect AvrPphB protease activity is conserved among diverse plant species and that the PBS1 decoy strategy is effective at introducing a novel disease resistance trait in soybean. The decoy strategy described herein should, therefore, be feasible in other crop plants given the conservation of PBS1. |
| 590 | |
▼a School code: 0093. |
| 650 | 4 |
▼a Molecular biology. |
| 650 | 4 |
▼a Plant Pathology. |
| 690 | |
▼a 0307 |
| 690 | |
▼a 0480 |
| 710 | 20 |
▼a Indiana University.
▼b Biology. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 80-12B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0093 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15491659
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |