| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000432651 |
| 005 | | 20200224133317 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781085686310 |
| 035 | |
▼a (MiAaPQ)AAI13901907 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Gauvin, Timothy Joseph. |
| 245 | 10 |
▼a Mechanisms Controlling Germ Plasm Enrichment during Asymmetric Cell Division in the C. elegans Embryo. |
| 260 | |
▼a [S.l.]:
▼b Dartmouth College.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 132 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-04, Section: B. |
| 500 | |
▼a Advisor: Griffin, Erik E. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Dartmouth College, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 506 | |
▼a This item must not be added to any third party search indexes. |
| 520 | |
▼a An important task for any embryo during development is to specify the germ cell for ensuring the survival of the species. The nematode, C. elegans, specifies the embryonic germline lineage through the asymmetric enrichment of germ plasm proteins. PIE-1 and POS-1 are two RNA binding proteins that localize to the germ plasm by two well-studied mechanisms: segregation and degradation. It is unknown if other mechanisms contribute to germline fate. Regulation of PIE-1 and POS-1 mobility are required for enrichment to the posterior in the zygote. Two factors that affect mobility are RNA binding and PLK-1 phosphorylation. A previous study has shown that PLK-1 phosphorylation increases POS-1 mobility in the anterior while RNA binding may decrease mobility in the posterior. Currently, there is no evidence to support the direct effects of phosphorylation on RNA binding.In the first part of my dissertation, I show by quantitative microscopy and photobleaching experiments that PIE-1 translation occurs specifically in the germline. To identify potential regulators, I screened ~249 genes by monitoring GFP levels at the 4 cell stage for a change in PIE-1 concentration. I identified two genes, Y-14 and MAG-1 that are part of the exon junction complex (EJC) as novel regulators of maternal translation. Neither gene exhibited zygotic translation. Future studies will identify potentials genes that regulate PIE-1 translation.PLK-1 phosphorylates POS-1 and consequently affects the mobility of POS-1 in the zygote. Based on this model, I developed protocols to purify POS-1 and its phosphorylation mutants using RNA binding assays to test oligomerization. By using fluorescence polarization (FP), the POS-1 zinc finger domain binds with the same affinity as POS-1 N-terminal truncation. However, we are not able to make any conclusions since POS-1 aggregates in a size exclusion column. Thus, we would need to find optimal conditions to prevent aggregation of POS-1 and then test if PLK-1 phosphorylation affects POS-1 RNA binding. |
| 590 | |
▼a School code: 0059. |
| 650 | 4 |
▼a Biology. |
| 650 | 4 |
▼a Developmental biology. |
| 690 | |
▼a 0306 |
| 690 | |
▼a 0758 |
| 710 | 20 |
▼a Dartmouth College.
▼b Biology. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-04B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0059 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15492330
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |