| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000433050 |
| 005 | | 20200225111632 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781088309223 |
| 035 | |
▼a (MiAaPQ)AAI13809426 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Marsh, Edward. |
| 245 | 14 |
▼a The Homeostasis of the Structure-Makers of Our Skin. |
| 260 | |
▼a [S.l.]:
▼b Yale University.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 93 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-03, Section: B. |
| 500 | |
▼a Advisor: Greco, Valentina. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Yale University, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 506 | |
▼a This item must not be added to any third party search indexes. |
| 520 | |
▼a Fibroblasts produce the matrices that give structure, strength, and elasticity to our organs, and these properties are essential to the function of those organs over our lifetimes. Despite several advances, the critical behaviors that fibroblasts utilize to maintain their homeostasis in vivo have remained unclear, and understanding the principles that guide this homeostasis has been the focus of my graduate work. By tracking the same skin fibroblasts in live mice, we discovered that fibroblast position is stable over an animal's lifetime, and that this stability is maintained despite the loss of neighboring fibroblasts. In contrast, fibroblast membranes are dynamic during homeostasis and extend to fill the space of lost neighboring fibroblasts in a modulator of actin dynamics, RHO GTPase Rac1, dependent manner. Positional stability is sustained during aging despite a progressive accumulation of gaps in fibroblast nuclei organization, while membrane occupancy continues to be maintained. Our ongoing work has identified contexts in which positional stability becomes less rigid, possibly resulting in increased regenerative properties, such as in either normal, or ectopically induced, neo-natal states. Finally, we developed a fibroblast cell culture approach to interrogate whether the fibroblast stability we observe at the tissue level during aging is also maintained at the subcellular level. Overall, my graduate work has defined positional stability and cell occupancy as key principles of skin fibroblast homeostasis in vivo, throughout the lifespan of mice, and identified membrane extension in the absence of migration as the core cellular mechanism to carry out these principles. |
| 590 | |
▼a School code: 0265. |
| 650 | 4 |
▼a Genetics. |
| 650 | 4 |
▼a Cellular biology. |
| 650 | 4 |
▼a Developmental biology. |
| 690 | |
▼a 0369 |
| 690 | |
▼a 0379 |
| 690 | |
▼a 0758 |
| 710 | 20 |
▼a Yale University.
▼b Genetics. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-03B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0265 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15490594
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1816162 |
| 991 | |
▼a E-BOOK |