| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000434115 |
| 005 | | 20200226141116 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781088353462 |
| 035 | |
▼a (MiAaPQ)AAI22585421 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 631.4 |
| 100 | 1 |
▼a Sharma, Kriti. |
| 245 | 10 |
▼a Illuminating Dark Matter: Light Microscopy and Raman Microspectroscopy Through Transparent Porous Media for Applications in Soil and Sediment Microbial Ecology. |
| 260 | |
▼a [S.l.]:
▼b The University of North Carolina at Chapel Hill.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 167 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-05, Section: B. |
| 500 | |
▼a Advisor: Shank, Elizabeth A. |
| 502 | 1 |
▼a Thesis (Ph.D.)--The University of North Carolina at Chapel Hill, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 520 | |
▼a Soils offer habitats to an unparalleled abundance and diversity of microorganisms, whose activities are critical to agriculture, ecosystem health, and biogeochemical cycling. A major barrier to understanding soil microbes within their habitats is the opacity of natural soils. Despite a long history of endeavors to visualize life in the soil, and promising advancements in this field, non-destructive approaches that allow dynamic insights into microbial life in soils are particularly lacking. Chapter 1 reviews this field and outlines the history and potential of optically transparent porous media as model soil systems amenable to non-destructive imaging of soil microorganisms within three-dimensional soil-like matrices.In Chapter 2, I introduce the utility of single-cell Raman spectroscopy (SCRS) for non-destructive stable isotope probing over time, particularly for monitoring the uptake of 13C by bacteria from complex natural polysaccharides. This spatially resolved and non-destructive approach allows us to ask the question, "Do bacterial biofilms allow bacteria to stick together in numbers large enough to initiate cooperative decomposition of necromass?'In Chapter 3, I assess the polymer Nafion and the crystal cryolite as substrates for optically transparent model soil systems called "transparent soil" (TS) microcosms. I find that both substrates are compatible with optical microscopy and enable growth, maintenance, and visualization of micron-sized bacteria in three-dimensional porous matrices over time. Both substrates are also compatible with SCRS, and enable stable isotope probing (SIP) using deuterium (D2O) as a non-destructive marker of microbial activity in situ, while cryolite-based microcosms also enable measurement of 13C label uptake in bacteria. I use D2O label tracing to show that bacterial cells attached to dead fungal hyphae within a Nafion matrix show more metabolic activity after a dry-wet cycle than cells far away from the fungal hyphae, corroborating the important role of fungi in facilitating survival of bacteria in the fluctuating conditions found in soils.In Chapter 4, I present a method for rapid and inexpensive manufacture of microfluidics devices that were used to construct TS microcosms at the lab bench. Chapter 5 summarizes the dissertation overall and offers suggestions for future research. |
| 590 | |
▼a School code: 0153. |
| 650 | 4 |
▼a Microbiology. |
| 650 | 4 |
▼a Ecology. |
| 650 | 4 |
▼a Soil sciences. |
| 690 | |
▼a 0410 |
| 690 | |
▼a 0329 |
| 690 | |
▼a 0481 |
| 710 | 20 |
▼a The University of North Carolina at Chapel Hill.
▼b Biology. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-05B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0153 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15492943
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |