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020 ▼a 9781085633925
035 ▼a (MiAaPQ)AAI27525358
035 ▼a (MiAaPQ)NCState_Univ18402036363
040 ▼a MiAaPQ ▼c MiAaPQ ▼d 247004
0820 ▼a 641
1001 ▼a Chilungo, Sarah.
24510 ▼a Bioaccessibility of Beta Carotene in Processed Products from Orange-Fleshed Sweetpotatoes.
260 ▼a [S.l.]: ▼b North Carolina State University., ▼c 2019.
260 1 ▼a Ann Arbor: ▼b ProQuest Dissertations & Theses, ▼c 2019.
300 ▼a 142 p.
500 ▼a Source: Dissertations Abstracts International, Volume: 81-03, Section: B.
500 ▼a Advisor: Harris, Gabriel
5021 ▼a Thesis (Ph.D.)--North Carolina State University, 2019.
506 ▼a This item must not be sold to any third party vendors.
520 ▼a Orange-Fleshed sweetpotatoes (OFSP) contain significant quantities of 棺-carotene, a precursor for vitamin A. The crop is being promoted to tackle vitamin A deficiency, a serious public health problem affecting children and pregnant/lactating women in sub-Saharan Africa. Significant studies have reported the efficacy of OFSP in improving serum retinol. The aim of the thesis was to evaluate the bioaccessibility of 棺-carotene in processed products from OFSP as affected by processing method and oil type. Bioaccessibility is the fraction or quantity of nutrients in food that is released from the matrix during digestion in the gastrointestinal tract and becomes available for absorption. Static in vitro digestion methods were used to determine carotene bioaccessibility. Bioconversion of 棺-carotene into VA and carotene retention stored in different packaging material were also investigated.This first study evaluated the effect of storage and packaging materials on carotene content, color and water activity of OFSP flours. Flours from Vita and Kabode OFSP genotypes were packed in aluminum foil laminate (AFL), high density polyethylene (HDPE) and Kraft paper and stored under light and dark conditions for 4 months. The highest carotenoid loss after 4 months was found in Kraft paper while AFL was the least. Similarly, flours in Kraft paper registered significant color value changes compared to flours in HDPE and AFL. Significant increase in water activity was observed in all packed samples and results were not dependent of storage environment. It is concluded that AFL with vacuum sealing is an appropriate packaging material due to better carotene retention, minimal color change and less water activity.Porridge and chapati were prepared with either puree or flour from the two sweetpotato genotypes. Digestive stability and bioaccessibility of the products were evaluated following in vitro methods. Beta-carotene digestive stability of porridge was significantly lower than chapatis. In the same line, 棺-carotene bioaccessibility was significantly lower for porridge than chapatis. It was also found that puree-based products registered low 棺-carotene bioaccessibility compared to flour-based products. For all the products, the study found no effect of sweetpotato genotype of 棺-carotene bioaccessibility while similar products had comparable carotene bioaccessibility. The findings confirm the effect of processing methods on carotene bioaccessibilty.Sunflower oil, margarine and beef fat were evaluated on their effect on 棺-carotene bioaccessibility. The amount of oil added was 10% (w/w) of chapati formulation. The results showed that digestive stability was non-significant among the products implying that oil type has no effect on digestive stability. Among the three oil types, sunflower oil had the highest carotene bioaccessibility followed by margarine while beef fat was the least. The low melting point of sunflower oil was the possible reason for high carotene bioaccessibility. Therefore, consumers should consider replacing margarine with sunflower oil due to low price and availability.The study further investigated the in vitro bioconversion efficiency of 棺-carotene into VA to determine VA value of OFSP supplemented porridge and chapati. When 棺-carotene extract was incubated with chicken intestinal mucosa post mitochondrial fractions, retinal (RAL) and retinoic acid (RA) were formed. No RAL and RA were formed when pure all-trans 棺-carotene standard was incubated without chicken intestinal mucosa post mitochondrial fraction. On average, the conversion ratio of 棺-carotene to RAL was 5:1. The results are of significance to people in developing countries who depend on plant food to meet their VA requirements. Further investigation of the study is suggested to validate the findings.
590 ▼a School code: 0155.
650 4 ▼a Food science.
690 ▼a 0359
71020 ▼a North Carolina State University.
7730 ▼t Dissertations Abstracts International ▼g 81-03B.
773 ▼t Dissertation Abstract International
790 ▼a 0155
791 ▼a Ph.D.
792 ▼a 2019
793 ▼a English
85640 ▼u http://www.riss.kr/pdu/ddodLink.do?id=T15494116 ▼n KERIS ▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다.
980 ▼a 202002 ▼f 2020
990 ▼a ***1008102
991 ▼a E-BOOK