| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000434325 |
| 005 | | 20200226145551 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781085644617 |
| 035 | |
▼a (MiAaPQ)AAI27528239 |
| 035 | |
▼a (MiAaPQ)NCState_Univ18402036801 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 630 |
| 100 | 1 |
▼a Beachler, Theresa Marie. |
| 245 | 10 |
▼a Metabolomic Profiling of Pregnant Mares with Experimental Ascending Placentitis. |
| 260 | |
▼a [S.l.]:
▼b North Carolina State University.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 185 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-03, Section: B. |
| 500 | |
▼a Advisor: Odle, Jack |
| 502 | 1 |
▼a Thesis (Ph.D.)--North Carolina State University, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 520 | |
▼a Equine ascending placentitis caused by migration of bacteria through the mare's reproductive tract remains a clinical challenge. Diagnosis is complicated by minimal to inconsistent clinical signs that are displayed in affected animals as well as the diagnostic modalities available for diagnosis early in the clinical course of disease. Currently, the diagnosis of ascending placentitis during pregnancy relies on a combination of clinical signs and transrectal ultrasonography to examine for thickening of the combined thickness of the uterus and placenta or the presence of placental separation near the level of the cervix. A host of other adjunctive diagnostic modalities such as hormonal profiling or the evaluation of markers of inflammation and infection such as serum amyloid A have been examined. Many of the changes noted, however, may also be detected in cases of systemic disease as well as conditions affecting other body systems or the fetoplacental unit in addition to ascending placentitis. Metabolomic profiling studies recently have been explored in both human medicine as well as veterinary medicine for the identification of biomarkers which may be used for diagnosing or identifying individuals at risk for developing a particular condition or disease. Therefore, the purpose of this research was to identify the metabolomic profile of biofluids from mares affected by experimental placentitis compared to that of healthy animals, with the goal of identifying individual metabolites or metabolite profiles that may be useful for identifying mares affected by the clinical disease.In order to examine this, one-dimensional and two-dimensional nuclear magneticresonance (NMR) spectroscopic based metabolomics was performed on collected plasma and allantoic fluid to identify and quantify metabolites present. An experimental model of Streptococcus equi subspecies zooepidemicus ascending placentitis was used to simulate disease in mares at 280 to 285 days of gestation. Plasma and allantoic fluid were serially collected from inoculated mares and healthy control animals at time points within the acute phase of infection until abortion, stillbirth, or foaling. Concurrently, whole blood lactate, serum amyloid A (SAA), and concentrations of progesterone, and estradiol-17棺 were collected along with the performance of transrectal and transabdominal ultrasonography at each time-point to compare the onset of metabolomic changes to those of commonly utilized diagnostic modalities. Surprisingly, metabolomic alterations within the allantoic fluid were not identified between healthy and inoculated mares prior to or after the diagnosis of ascending placentitis. In contrast, in plasma two phases of metabolic changes were noted after experimental inoculation and infection. An immediate rise in the concentration of metabolites involved in energy, nitrogen, hydrogen, and oxygen metabolism were seen within four hours of inoculation and was followed by a decrease in metabolite concentrations involved in energy and nitrogen metabolism by day four coinciding with the ultrasonographic diagnosis of placentitis and prior to elevations in the systemic marker of inflammation, serum amyloid A. In summary, these studies serve to identify and expand the metabolomic profile of equine allantoic fluid and plasma respectively. Further research is needed to determine if these metabolic alterations could serve as a diagnostic target for the confirmation of disease in mares affected by experimental placentitis as well as in natural cases of infection. |
| 590 | |
▼a School code: 0155. |
| 650 | 4 |
▼a Biomedical engineering. |
| 650 | 4 |
▼a Animal sciences. |
| 650 | 4 |
▼a Veterinary services. |
| 690 | |
▼a 0475 |
| 690 | |
▼a 0541 |
| 690 | |
▼a 0778 |
| 710 | 20 |
▼a North Carolina State University. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-03B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0155 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15494122
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |