| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000434808 |
| 005 | | 20200227105457 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781392880005 |
| 035 | |
▼a (MiAaPQ)AAI22623800 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 610 |
| 100 | 1 |
▼a Karas, Christina E. |
| 245 | 10 |
▼a A Strategy for Combinatorial Cavity Design in De Novo Proteins. |
| 260 | |
▼a [S.l.]:
▼b Princeton University.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 175 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-06, Section: B. |
| 500 | |
▼a Advisor: Hecht, Michael H. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Princeton University, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 520 | |
▼a Protein sequence space is vast |
| 520 | |
▼a 6 unique proteins, assembled from degenerate oligonucleotides. The third and fourth parts of this work cover the screening effort for a range of activities, both in vitro and in vivo. I found that this collection binds heme readily, leading to abundant peroxidase activity. Hits for lipase and phosphatase activity were also detected.This thesis details the development of a new strategy for creating de novo sequences geared toward function rather than structure. Following my work, these library design principles are being applied to SynF4, a de novo enterobactin esterase, whose structure was recently solved. By diversifying the cavity of SynF4, we hope to create a new family of de novo enzymes. Altogether, this approach represents a step towards creating artificial proteomes capable of carrying out essential biological roles. |
| 590 | |
▼a School code: 0181. |
| 650 | 4 |
▼a Molecular biology. |
| 650 | 4 |
▼a Chemistry. |
| 650 | 4 |
▼a Bioengineering. |
| 690 | |
▼a 0307 |
| 690 | |
▼a 0485 |
| 690 | |
▼a 0202 |
| 710 | 20 |
▼a Princeton University.
▼b Molecular Biology. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-06B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0181 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15494024
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |