| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000434841 |
| 005 | | 20200227110136 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781392678923 |
| 035 | |
▼a (MiAaPQ)AAI27543361 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 575 |
| 100 | 1 |
▼a Fritts, Ryan Kyle. |
| 245 | 14 |
▼a The Physiology, Mechanisms, and Evolution of Bacterial Cross-Feeding and Nutrient Acquisition. |
| 260 | |
▼a [S.l.]:
▼b Indiana University.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 194 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-05, Section: B. |
| 500 | |
▼a Advisor: McKinlay, Jake B. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Indiana University, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 520 | |
▼a Microbes inhabit nearly every environment on Earth. Across these diverse ecosystems, microbes typically exist as members of highly interactive communities. Within microbial communities, there is often intense competition for nutrients. One prevalent way in which nutrients are acquired is through metabolite exchange, also called cross-feeding. Although it is known that cross-feeding is ubiquitous, it is less well understood how cross-feeding interactions arise, especially in cases where costly metabolites are exchanged. To address this, I experimentally evolved a synthetic bacterial community comprised of Rhodopseudomonas palustris and Escherichia coli. I demonstrated that these two bacteria can rapidly adapt to coexist by bi-directionally exchanging essential nutrients. Furthermore, I showed that when cross-feeding of costly ammonium spontaneously evolves, it is the recipient E. coli, rather than the producer R. palustris, driving the emergence of cross-feeding. I subsequently elucidated the molecular mechanism underlying enhanced ammonium acquisition in E. coli, which enables nascent cross-feeding. I also examined the physiological and evolutionary responses of R. palustris to growth in an obligate cross-feeding partnership with E. coli. Under coculture conditions, loss-of-function mutations in a putative nitrogen metabolism regulatory system of R. palustris appear to be adaptive for coexistence with E. coli. Further focusing on R. palustris, I surveyed how the multicellular behavior biofilm formation varies in R. palustris across its metabolic lifestyles by altering nutrient and resource availability. I determined that synthesis of unipolar polysaccharide (UPP) is critical for R. palustris adhesion and that biofilm formation is elevated during nitrogen and phosphorus limitation, potentially as a nutrient acquisition strategy. Overall, my dissertation work integrates metabolic, biochemical, genetic, genomic, cellular, and evolutionary techniques and approaches to provide insights into the physiology, mechanisms, and evolution of bacterial cross-feeding and nutrient acquisition. |
| 590 | |
▼a School code: 0093. |
| 650 | 4 |
▼a Microbiology. |
| 650 | 4 |
▼a Evolution & development. |
| 650 | 4 |
▼a Genetics. |
| 690 | |
▼a 0410 |
| 690 | |
▼a 0412 |
| 690 | |
▼a 0369 |
| 710 | 20 |
▼a Indiana University.
▼b Biology. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-05B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0093 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15494455
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |