| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000436354 |
| 005 | | 20200228141613 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781085787680 |
| 035 | |
▼a (MiAaPQ)AAI13806383 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Hu, Yuhan. |
| 245 | 10 |
▼a Regulation of Microtubule Dynamics via Abl2 Binding. |
| 260 | |
▼a [S.l.]:
▼b Yale University.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 114 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-03, Section: B. |
| 500 | |
▼a Advisor: Koleske, Anthony J. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Yale University, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 506 | |
▼a This item must not be added to any third party search indexes. |
| 520 | |
▼a Abl family kinases are believed to play as central signaling nodes to coordinate microtubule dynamics by transducing signals from upstream guidance receptors to downstream microtubule-associated proteins. Here, I report a novel microtubule dynamics regulation mechanism via direct binding of Abl2. In this study, I discovered that Abl2 directly binds microtubules and regulate microtubule dynamics. In a purified recombinant system, I identified a central proline-rich region inside Abl2 that is sufficient and necessary to bind to microtubules, and the interaction between Abl2 and microtubules is electrostatically mediated by the COOH-terminal acidic tubulin tail. Using in vitro single-filament microscopy, I found that Abl2 stably bound onto microtubule lattice and regulated microtubule dynamics by increasing elongation rate, reducing shortening rate, and decreasing catastrophe frequency. In cells, when Abl2 was knocked out microtubule growth rate was significant reduced and re-expressing Abl2 successfully rescued the microtubule growth defect. These and other data suggest a model where Abl2 act as a microtubule regulator at the cell periphery. |
| 590 | |
▼a School code: 0265. |
| 650 | 4 |
▼a Biology. |
| 650 | 4 |
▼a Biochemistry. |
| 650 | 4 |
▼a Cellular biology. |
| 690 | |
▼a 0306 |
| 690 | |
▼a 0487 |
| 690 | |
▼a 0379 |
| 710 | 20 |
▼a Yale University.
▼b Cell Biology. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-03B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0265 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15490481
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |