| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000436355 |
| 005 | | 20200228141619 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781085777407 |
| 035 | |
▼a (MiAaPQ)AAI13806441 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a East, Kyle William. |
| 245 | 14 |
▼a The Role of Protein-Nucleic Acid Interactions in the Function and Fidelity of DNA Polymerase 棺 and CRISPR Cas9 Monitored by NMR. |
| 260 | |
▼a [S.l.]:
▼b Yale University.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 148 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-03, Section: B. |
| 500 | |
▼a Advisor: Loria, J. Patrick. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Yale University, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 506 | |
▼a This item must not be added to any third party search indexes. |
| 520 | |
▼a Protein-nucleic acid interactions are one of the most important processes required for life on earth. These interactions make up the core of the central dogma of molecular biology first published by Francis Crick in 1958. For over six decades, the interactions of proteins with DNA and RNA have been heavily studied in order to better understand how the proteome interacts with the genome and nucleic acid polymers. The complexity of these interactions is best highlighted by the thousands of proteins that interact with genomic DNA and various RNAs. These protein-nucleic acid interactions are required for the integrity of the genome and the stability of the processes required by the central dogma. The integrity of genomic DNA requires the fidelity of the DNA polymerases that copy and repair the millions of DNA base pairs per cell. One polymerase that repair DNA in humans is DNA polymerase 棺. Studies of DNA polymerase 棺 (pol 棺) have shown that the interactions of pol 棺 with the target DNA are tightly regulated to maximize the fidelity of the enzyme. The wild type enzyme was monitored using NMR spectroscopy in order to study the effects of the four template nucleotides (adenosine, guanosine, thymine, and cytosine) have on the structure and dynamics of the enzyme. The wild type enzyme was shown to bind independently of the nucleotide |
| 590 | |
▼a School code: 0265. |
| 650 | 4 |
▼a Chemistry. |
| 650 | 4 |
▼a Biophysics. |
| 690 | |
▼a 0485 |
| 690 | |
▼a 0786 |
| 710 | 20 |
▼a Yale University.
▼b Chemistry. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-03B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0265 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15490482
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |