| LDR | | 00000nam u2200205 4500 |
| 001 | | 000000436747 |
| 005 | | 20200228151802 |
| 008 | | 200131s2019 ||||||||||||||||| ||eng d |
| 020 | |
▼a 9781088320280 |
| 035 | |
▼a (MiAaPQ)AAI13809639 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 247004 |
| 082 | 0 |
▼a 581 |
| 100 | 1 |
▼a Miller, Jimi Craig. |
| 245 | 10 |
▼a Canonical and Non-Canonical Plant Heterotrimeric G Proteins in Plant Immunity. |
| 260 | |
▼a [S.l.]:
▼b Yale University.,
▼c 2019. |
| 260 | 1 |
▼a Ann Arbor:
▼b ProQuest Dissertations & Theses,
▼c 2019. |
| 300 | |
▼a 133 p. |
| 500 | |
▼a Source: Dissertations Abstracts International, Volume: 81-04, Section: B. |
| 500 | |
▼a Advisor: Clay, Nicole K. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Yale University, 2019. |
| 506 | |
▼a This item must not be sold to any third party vendors. |
| 506 | |
▼a This item must not be added to any third party search indexes. |
| 520 | |
▼a Plants contain an innate immune system, which relies on the cell surface receptor FLAGELLIN-SENSITIVE 2 (FLS2) binding to the conserved bacterial molecule flagellin, to initiate immune signaling. The signal from activated FLS2 is transduced by a heterotrimeric G protein complex. This complex consists of a G慣, G棺, and G款 subunit and co-localizes with FLS2 at the plasma membrane. It remains unknown whether the heterotrimeric G proteins have additional functions away from signal transduction at the plasma membrane or if additional undiscovered heterotrimeric G subunits exist. Here, I show that two heterotrimeric G protein components function at the endoplasmic reticulum to promote the stability of FLS2. Specifically, the G棺款 heterodimer inhibits the autophagic degradation of FLS2, resulting in stable FLS2 protein levels. In addition to identifying novel functions of the G棺款 heterodimer that promote immune signaling, I also found two potential non-canonical G棺 subunits that negatively regulate immune signaling. I identified two genes, DEFENSE REGULATED WD40-REPEAT 1 and 2 (DRW1 and DRW2), that encode potential non-canonical G棺 subunits. Their predicted protein structure is similar to that of the canonical G棺 subunit ARABIDOPSIS GTP BINDING PROTEIN 棺-1 SUBUNIT (AGB1). Mutations in both DRW1 and DRW2 results in an increased resistance to both bacterial and fungal pathogen infections, indicating that these potential noncanonical G棺 subunits negatively regulate immune signaling. My studies show first that the heterotrimeric G proteins have novel functions away from the plasma membrane.Second, I have identified new plant heterotrimeric G protein components that are involved in immunity. These results provide insights into how plant heterotrimeric G proteins function independently of their canonical role in signal transduction at the plasma membrane. Additionally, my results provide a foundation for future studies to further elucidate plant immune signaling pathways, and identifies proteins for researchers to manipulate genetically to help bolster immunity against pathogens in crops. |
| 590 | |
▼a School code: 0265. |
| 650 | 4 |
▼a Plant sciences. |
| 650 | 4 |
▼a Plant pathology. |
| 690 | |
▼a 0479 |
| 690 | |
▼a 0480 |
| 710 | 20 |
▼a Yale University.
▼b Molecular Biophysics and Biochemistry. |
| 773 | 0 |
▼t Dissertations Abstracts International
▼g 81-04B. |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0265 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2019 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15490604
▼n KERIS
▼z 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
| 980 | |
▼a 202002
▼f 2020 |
| 990 | |
▼a ***1008102 |
| 991 | |
▼a E-BOOK |