CONTENTS Contributors to Volume 2 ... ⅸ Preface ... xiii Volumes in Series ... xv Section I General Approaches to Preparation of Cell Cultures 1. Multiple Approaches to Brain Culture / Cheryl F. Dreyfus ; Ira B. Black ... 3 2. Culture and Use of Primary and Clonal Neural Cells / Stephen D. Skaper ; Laura Facci ; Daria Milani ; ... 17 3. Generation of Astrocyte Cultures from Normal and Neoplastic Central Nervous System / Sean Murphy ... 33 4. Isolation of Astrocytes and Schwann Cells for Culture / Ramon Lim ; E. Peter Bosch ... 47 5. Culture Methods for Oligodendrocyte Cell Lines and Oligodendrocyte-Type 2 Astrocyte Lineage Cells / Jane E. Bottenstein ; Samuel F. Hunter ... 56 6. Neuropharmacological Analyses in Organotypic Cultures of Spinal Cord and Dorsal Root Ganglia / Stanley M. Crain ... 75 7. Isolation, Culture, and Use of Viable Central Nervous System Perikarya / Gary R. Dutton ... 87 8. Cerebral Cortical Neurons in Primary Culture "and Application to Neuropharmacological Studies / Kinya Kuriyama ; Seitaro Ohkuma ... 103 Section II Purification of Cell Types 9. Preparation of Highly Purified Populations of Neurons, Astrocytes, and Otigodendrocytes / Russell P. Saneto ... 119 10. Preparation, Enrichment, and Growth of Purified Cultures of Neurons and Photoreceptors from Chick Embryos and from Normal and Mutant Mice / Ruben Adler ... 134 Section III Bulk Isolation 11. Bulk Isolation of Mouse Oligodendrocytes by Gradient Centrifugations / Liane Bologa ; Norbert Herschkowitz ... 153 12. Bulk Isolation and Culture of Oligodendroglia from Mature Brain / William T. Norton ; Muhammad Farooq ... 171 Section IV Special Approaches 13. Measurement of Pulsatile Hormone Release from Perifused Pituitary Cells Immobilized on Microcarriers / James R. Hansen ; P. Michael Conn ... 181 14. Cultivation of Nerve and Muscle Cells on Microcarriers / Abraham Shahar ... 195 15. Monolayer and Three-Dimensional Culture of Rat and Human Central Nervous System: Normal and Malignant Cells and Their Interactions / Ole Didrik Laerum ; Rolf Bjerkvig ... 210 16. Brain Cell Culture in Rotation-Mediated Aggregates / Paul C. Goldsmith ; Michael E. Berens ... 236 17. Microcultures of Dissociated Primary Contral Nervous System Neurons / Efrain C. Azmitia ... 263 18. Long-Term Organotypic Culture of Central Nervous System in Maximow Assemblies / C. Dominique Toran-Allerand ... 275 Section V Functional Assessment 19. Preparation and Use of Morphological Variants to Investigate Neuropeptide Action / John S. Ramsdell ... 299 20. In Vivo Tissue Culture: Use of Ectopic Anterior Pituitary Gland to Study Neuropeptide and Steroid Actions on Adenohypophysis / Frank J. Strobl ; Jon E. Levine ... 316 21. Evaluation of Catecholaminergic Activity in Hypothalamic Cultures / Barbara A. Bennett ; David K. Sundberg ; Mariana ... 330 22. Receptors for Bombesin/Gastrin-Releasing Peptide and Epidermal Growth Factor on Central Nervous System Cells / Terry W. Moody ; Julie Staley ; Mei Lee ; Richard ... 341 23. Use of Hypothalamic Cell Cultures to Study Role of Diffusible Factors in Phenotypic Expression of Central Nervous System Neurons / Andr e ´ e Tixier-Vidal ; Annie Faivre-Bauman ... 355 Section VI Cell Lineage 24. Microinjectable Probes for Tracing Cell Lineage in Development / Duncan K. Stuart ; Steven A. Torrence ; Gunther S ... 375 25. Methods for Clonal Analysis and Tracing Cell Lineages of Vertebrate Central Nervous System / Marcus Jacobson ... 393 Index ... 407