제1부 Genetics in cardiovascular research 1. Detection of genetic variants by PCR-SSCP / 박현영[연세의대] ... 3 2. Analysis of genetic variants by single base extension / 이종은[(주) DNA 링크] ... 13 3. Gene diagnosis using DNA chip / 이득주[(주)마이진] ... 20 제2부 Expression analysis of gene and protein 1. RNA extraction and RT-PCR / 김재우[연세의대] ... 35 2. Real time reverse-transcriptase(RT) PCR / 김건홍[연세의대] ... 48 3. Isolation of protein and Western blotting / 정지형[연세의대] ... 62 4. Protein analysis by proteomics 2-Dimensional Gel Electrophoresis / 정광회[연세의대] ... 74 제3부 Molecular methods to study in cardiovascular tissue 1. In Situ Hybridization / 성제경[연세의대] ... 87 2. Immunohistochemical staining / 정익모[이화의대] ... 94 3. Detection of apoptosis in Cardiovascular cells/tissue / 양현원[연세의대] ... 100 제4부 Animal models for cardiovascular research 1. Animal models for restenosis and local drug delivery / 최동훈[연세의대] ... 115 2. Animal models for heart failure / 유경종[연세의대] ... 124 3. Rabbit hindlimb ischemic model and evaluation of angiogenesis / 이병권[인제의대] ... 146 4. Porcine ischemic model using ameroid ring / 홍유선[연세의대] ... 154