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Delineating the Role of Oxygen in Biofilm Formation in Escherichia Coli Urinary Tract Isolates
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Delineating the Role of Oxygen in Biofilm Formation in Escherichia Coli Urinary Tract Isolates. |
| 개인저자 | Eberly, Allison Rae. |
| 단체저자명 | Vanderbilt University. Microbiology and Immunology. |
| 발행사항 | [S.l.]: Vanderbilt University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 224 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-04B. Dissertation Abstract International |
| ISBN | 9781088318393 |
| 학위논문주기 | Thesis (Ph.D.)--Vanderbilt University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Advisor: Hadjifrangiskou, Maria. |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | Urinary tract infections (UTIs) are very common, the disease severity can encompass a broad range of clinical diagnoses, including acute cystitis, pyelonephritis, catheter-associated infection, and asymptomatic bacteriuria. UPEC is a Gram-negative facultative anaerobe that forms multi-cellular communities known as biofilms on the surface of catheter material, urinary tract tissues, and within bladder epithelial cells. The formation of biofilms greatly impedes treatment efforts by protecting resident bacteria from external stressors, such as the innate immune response and antibiotic treatment. Biofilm formation by UPEC was studied under different oxygen concentrations and resulted in reduction in oxygen concentration from atmospheric to anoxic conditions coincided with gradual reduction in biofilm formation, and the addition of alternative terminal electron acceptors typically used by E. coli was not sufficient in restoring biofilm formation. Furthermore, there is no pathogenetic signature that predicts if a UPEC strain is asymptomatic or symptom-causing. Furthermore, no studies have examined whether there is a correlation between the biofilm-forming properties of uropathogenic and asymptomatic E. coli isolates. A retrospective study was performed in collaboration with the Vanderbilt Clinical Microbiology Laboratory in which over 300 isolates were collected from monoculture, E. coli-positive urine specimens with corresponding de-identified patient data. The isolates were blindly tested for in vitro extracellular biofilm formation during growth in laboratory media. These assays demonstrated similar variability in the biofilm forming abilities, indicating that there is no specific correlation between biofilm formation and strains that cause symptomatic UTI. However, preliminary metabolomics data of these clinical isolates show metabolic differences between asymptomatic and symptom-causing isolates. |
| 일반주제명 | Microbiology. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
