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CONTENTS
Preface ... ⅲ
Introduction ... ⅸ
1. Basic Components ... 1
Nomenclature of Compounds ... 1
Structure ... 4
Shorthand Notation ... 6
References ... 11
2. The Cell ... 13
The ...
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목차 전체
CONTENTS
Preface ... ⅲ
Introduction ... ⅸ
1. Basic Components ... 1
Nomenclature of Compounds ... 1
Structure ... 4
Shorthand Notation ... 6
References ... 11
2. The Cell ... 13
The Prokaryotic Cell ... 14
The Eukaryotic Cell ... 22
References ... 26
3. DNA, RNA, and Genes ... 27
History of DNA ... 29
Genes ... 30
DNA ... 31
RNA ... 37
Chemical Synthesis of DNA ... 44
References ... 55
4. Protein Synthesis ... 57
Proteins ... 57
Differences in Prokaryotic and Eukaryotic DNA and RNA ... 66
References ... 72
5. Enzymes ... 75
Nucleases ... 76
Site-Specific Restriction Endonucleases ... 77
Enzymes Used in Recombinant DNA Technology ... 77
Cleaving of RNA ... 81
References ... 81
6. Plasmids, Viruses, and Microbial Hosts-Vectors ... 83
Plasmids ... 83
Chromatographic Plasmid Isolation ... 89
Viruses ... 94
Viruses Infecting Eukaryotes ... 102
Microbial Hosts ... 103
Hosts ... 104
Availability of Biologicals ... 108
References ... 108
7. Recombinant Techniques ... 111
Basic Concepts ... 111
Generalized Scheme ... 112
Cutting Sites of Vectors ... 116
DNA Denaturation and Renaturation ... 117
Cleaving of DNA ... 118
Creation of Cohesive Ends ... 120
Recombining DNA Fragments ... 122
Bacterial Hosts Cloning Vehicles ... 127
Cloning ... 127
The Cloning Process ... 127
Specific Clones ... 129
Cloning in Mammalian Cells ... 131
Commercialization ... 134
References ... 136
8. Nucleotide Sequencing and Hybridizing ... 139
The Sequencing Gel ... 140
Sequencing Methods ... 140
Specific Methods ... 141
Chemical Sequencing Method ... 145
References ... 153
9. Plant Genetics ... 155
Gene Transfer Procedures ... 155
References ... 161
10. Genetic Engineering Activities ... 165
Fields of Endeavor ... 165
Somatostatin ... 166
Insulin ... 170
Commercial Synthesis of Human Insulin ... 171
Human Growth Hormone (HGH) ... 174
Interferons ... 183
Superbug ... 192
Creation of Other Synthetic Recombinants ... 192
Antihemophilic Agents ... 194
Synthetic Vaccines ... 194
Nonrecombinant Genetic Engineering ... 197
Applications of Monoclonal Antibodies ... 203
Fact or Fiction? ... 204
Genetic Engineering Extended ... 205
Addendum ... 205
References ... 205
11. Technology and Design ... 209
Technology Aspects ... 209
Technology : Uboratory Scale ... 210
Design Considerations ... 211
Design Classification ... 213
Equipment Design Features ... 216
Addendum ... 224
References ... 224
12. Techniques with Mammalian Cells ... 227
Gene Introduction to a Mammalian Cell ... 227
Human O-Globulin Gene in a Mammal ... 229
Human Genetics ... 229
Gene Therapy ... 235
References ... 242
13. Precautions and Regulations ... 243
Patent Situation ... 244
Economic Aspects ... 244
Documentation ... 245
Supervision Concerns ... 245
Epilogue ... 245
References ... 246
14. Update ... 247
Review Type Publications ... 247
Current Literature ... 248
Basic Studies ... 249
Plant Genetics ... 251
Medicines and Medical Applications ... 252
Technology ... 257
References ... 258
Appendices ... 263
Index ... 283
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