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CONTENTS
Contributors to Volume 2 ... ⅸ
Preface ... xiii
Volumes in Series ... xv
Section I General Approaches to Preparation of Cell Cultures
1. Multiple Approaches to Brain Culture / Cheryl F....
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CONTENTS
Contributors to Volume 2 ... ⅸ
Preface ... xiii
Volumes in Series ... xv
Section I General Approaches to Preparation of Cell Cultures
1. Multiple Approaches to Brain Culture / Cheryl F. Dreyfus ; Ira B. Black ... 3
2. Culture and Use of Primary and Clonal Neural Cells / Stephen D. Skaper ; Laura Facci ; Daria Milani ; ... 17
3. Generation of Astrocyte Cultures from Normal and Neoplastic Central Nervous System / Sean Murphy ... 33
4. Isolation of Astrocytes and Schwann Cells for Culture / Ramon Lim ; E. Peter Bosch ... 47
5. Culture Methods for Oligodendrocyte Cell Lines and Oligodendrocyte-Type 2 Astrocyte Lineage Cells / Jane E. Bottenstein ; Samuel F. Hunter ... 56
6. Neuropharmacological Analyses in Organotypic Cultures of Spinal Cord and Dorsal Root Ganglia / Stanley M. Crain ... 75
7. Isolation, Culture, and Use of Viable Central Nervous System Perikarya / Gary R. Dutton ... 87
8. Cerebral Cortical Neurons in Primary Culture "and Application to Neuropharmacological Studies / Kinya Kuriyama ; Seitaro Ohkuma ... 103
Section II Purification of Cell Types
9. Preparation of Highly Purified Populations of Neurons, Astrocytes, and Otigodendrocytes / Russell P. Saneto ... 119
10. Preparation, Enrichment, and Growth of Purified Cultures of Neurons and Photoreceptors from Chick Embryos and from Normal and Mutant Mice / Ruben Adler ... 134
Section III Bulk Isolation
11. Bulk Isolation of Mouse Oligodendrocytes by Gradient Centrifugations / Liane Bologa ; Norbert Herschkowitz ... 153
12. Bulk Isolation and Culture of Oligodendroglia from Mature Brain / William T. Norton ; Muhammad Farooq ... 171
Section IV Special Approaches
13. Measurement of Pulsatile Hormone Release from Perifused Pituitary Cells Immobilized on Microcarriers / James R. Hansen ; P. Michael Conn ... 181
14. Cultivation of Nerve and Muscle Cells on Microcarriers / Abraham Shahar ... 195
15. Monolayer and Three-Dimensional Culture of Rat and Human Central Nervous System: Normal and Malignant Cells and Their Interactions / Ole Didrik Laerum ; Rolf Bjerkvig ... 210
16. Brain Cell Culture in Rotation-Mediated Aggregates / Paul C. Goldsmith ; Michael E. Berens ... 236
17. Microcultures of Dissociated Primary Contral Nervous System Neurons / Efrain C. Azmitia ... 263
18. Long-Term Organotypic Culture of Central Nervous System in Maximow Assemblies / C. Dominique Toran-Allerand ... 275
Section V Functional Assessment
19. Preparation and Use of Morphological Variants to Investigate Neuropeptide Action / John S. Ramsdell ... 299
20. In Vivo Tissue Culture: Use of Ectopic Anterior Pituitary Gland to Study Neuropeptide and Steroid Actions on Adenohypophysis / Frank J. Strobl ; Jon E. Levine ... 316
21. Evaluation of Catecholaminergic Activity in Hypothalamic Cultures / Barbara A. Bennett ; David K. Sundberg ; Mariana ... 330
22. Receptors for Bombesin/Gastrin-Releasing Peptide and Epidermal Growth Factor on Central Nervous System Cells / Terry W. Moody ; Julie Staley ; Mei Lee ; Richard ... 341
23. Use of Hypothalamic Cell Cultures to Study Role of Diffusible Factors in Phenotypic Expression of Central Nervous System Neurons / Andr e ´ e Tixier-Vidal ; Annie Faivre-Bauman ... 355
Section VI Cell Lineage
24. Microinjectable Probes for Tracing Cell Lineage in Development / Duncan K. Stuart ; Steven A. Torrence ; Gunther S ... 375
25. Methods for Clonal Analysis and Tracing Cell Lineages of Vertebrate Central Nervous System / Marcus Jacobson ... 393
Index ... 407
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