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Inteins as Biomolecular Switches: Applications in Drug Discovery, Protein Purification, and Stress Response
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Inteins as Biomolecular Switches: Applications in Drug Discovery, Protein Purification, and Stress Response. |
| 개인저자 | Pearson, C. Seth. |
| 단체저자명 | Rensselaer Polytechnic Institute. Chemical Engineering. |
| 발행사항 | [S.l.]: Rensselaer Polytechnic Institute., 2018. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2018. |
| 형태사항 | 102 p. |
| 기본자료 저록 | Dissertation Abstracts International 79-12B(E). Dissertation Abstract International |
| ISBN | 9780438206229 |
| 학위논문주기 | Thesis (Ph.D.)--Rensselaer Polytechnic Institute, 2018. |
| 일반주기 |
Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B.
Advisers: Georges Belfort |
| 요약 | Inteins are protein elements that autocatalytically excise themselves from a host protein in a process called protein splicing. The two flanking sequences of the host protein, called the N- and C-exteins, are subsequently ligated with a native p |
| 요약 | During this process, the intein breaks two peptide bonds and forms a third. Taking advantage of the intein's ability to catalyze these reactions, both within the natural host as well as in vitro for biotechnological applications, provides an att |
| 요약 | Our work focuses on two of the three inteins found in the human pathogen Mycobacterium tuberculosis (Mtu), RecA and SufB. The intein-interrupted proteins are important for the viability of Mtu. Preventing intein excision, and thus the formation |
| 요약 | Turning to methods of control for in vitro applications, we introduced a mutation in Mtu RecA that causes the intein to spontaneously form a thiazoline ring at the N-terminal splicing junction. While present, the thiazoline ring prevents any fur |
| 요약 | The final aspect of our work is based on the intriguing hypothesis that inteins are not simply parasitic elements, but offer a beneficial protein-level control of host protein function. We demonstrated that Mtu SufB activity is inhibited in vivo |
| 요약 | Overall, this work investigates strategies for controlling inteins in the realms of drug development, protein purification, and host regulation and stress response. |
| 일반주제명 | Chemical engineering. Biochemistry. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
