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Toxicity Mediated by Seed-Dependent Off-Target Effects in RNA Interference
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Toxicity Mediated by Seed-Dependent Off-Target Effects in RNA Interference. |
| 개인저자 | Putzbach, William. |
| 단체저자명 | Northwestern University. Life Sciences. |
| 발행사항 | [S.l.]: Northwestern University., 2018. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2018. |
| 형태사항 | 261 p. |
| 기본자료 저록 | Dissertation Abstracts International 79-11B(E). Dissertation Abstract International |
| ISBN | 9780438116894 |
| 학위논문주기 | Thesis (Ph.D.)--Northwestern University, 2018. |
| 일반주기 |
Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B.
Adviser: Marcus E. Peter. |
| 요약 | Post-transcriptional gene silencing (PTGS) and, more specifically, RNA interference (RNAi) include the processes by which a small double-stranded RNA, 19 to 22 nucleotides (nts) long, negatively regulates the expression and/or translatability of |
| 요약 | Non-overlapping siRNAs that specifically and potently target the same gene would be predicted to evoke the same phenotypic change, provided the intended target performs an overt function. However, non-overlapping siRNAs with different seed seque |
| 요약 | Indeed, over 80% of commercially available siRNAs and short hairpin (sh)RNAs derived from CD95/CD95L mRNA sequences are toxic to cancer cells in the absence of the intended target site in these two genes, demonstrating this toxicity is independe |
| 요약 | These nonredundant CD95/CD95L-derived si/shRNAs all induce a novel form of cancer cell death, with distinct biochemical and morphological features best described as simultaneous activation of multiple death pathways. Interestingly, these toxic s |
| 요약 | The abundance of toxic shRNA sequences derived from the CD95L ORF, as identified in the screen, suggested the full-length mRNA may exhibit functionality independent of its role in translating for CD95L protein. Indeed, expression of wild type (W |
| 일반주제명 | Cellular biology. Biology. |
| 언어 | 영어 |
| 바로가기 | 
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