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A New Biophysical Model for Translation Elongation to Predict and Optimize Heterologous Protein Production in Escherichia coli
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | A New Biophysical Model for Translation Elongation to Predict and Optimize Heterologous Protein Production in Escherichia coli. |
| 개인저자 | Vu, Scott Khoi. |
| 단체저자명 | North Carolina State University. Biomedical Engineering. |
| 발행사항 | [S.l.]: North Carolina State University., 2015. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2015. |
| 형태사항 | 281 p. |
| 기본자료 저록 | Dissertation Abstracts International 79-12B(E). Dissertation Abstract International |
| ISBN | 9780438284494 |
| 학위논문주기 | Thesis (Ph.D.)--North Carolina State University, 2015. |
| 일반주기 |
Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B.
Advisers: Donald L. Bitzer |
| 요약 | Translation is a biological process in which an organism produces specific polypeptides that fold into functional proteins. Heterologous protein production uses translation to synthesize proteins that are not normally expressed in production org |
| 요약 | A new computational-based biophysical model for translation elongation has been developed that provides: a) new insights into the mechanisms behind translation, e.g. elucidating programmed frameshifts prfB and dnaX, and b) a new approach to gene |
| 요약 | The model's predictive and optimization accuracy were verified by optimizing and expressing five model genes: gst (glutathione-S-transferase), pf0132 (alpha-glucosidase), clju_c11880 (alcohol dehydrogenase), bif1 (beta-galactosidase), and rt8_0 |
| 요약 | Based on the results presented in this dissertation and the literature published, the author contends that the determinants of protein yield from translation elongation are not just codon bias, but, rather, the convolution between aminoacyl-tRNA |
| 일반주제명 | Biomedical engineering. Biophysics. Molecular biology. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
