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Methodologies for Metabolomics [electronic resource] Experimental Strategies and Techniques
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| 자료유형 | 단행본 |
|---|---|
| 서명/저자사항 | Methodologies for Metabolomics : Experimental Strategies and Techniques. [electronic resource]. |
| 개인저자 | Lutz, Norbert. Sweedler, Jonathan, Wevers, Ron, |
| 발행사항 | Cambridge: Cambridge University Press, 2012. |
| 형태사항 | 1 online resource (642 pages). |
| 기타형태 저록 | Print version: Lutz, Norbert. Methodologies for Metabolomics : Experimental Strategies and Techniques. Cambridge : Cambridge University Press, ©2012 9780521765909 |
| ISBN | 9781139615334 1139615335 9781283870375 1283870371 9780511996634 0511996632 9780521765909 0521765900 9781139624633 1139624636 |
| 일반주기 |
2. Considerations in the Growth, Harvest, Preparation, and Extraction of Plant Materials.
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| 서지주기 | Includes bibliographical references and index. |
| 내용주기 | Contributors; Section 1 Basic Methodological Strategies in Metabolomic Research; 1 Exploring the Human Metabolome by Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry; 1. Brief History of Human Metabolomics; 2. Sample Preparation for Human Metabolomic Studies; 3. Human Metabolite Identification by Nuclear Magnetic Resonance Spectroscopy; 4. Human Metabolite Identification by Gas Chromatography-Mass Spectrometry; 5. Human Metabolite Identification by Liquid Chromatography-Mass Spectrometry; 6. Comparison of Technologies; 7. Conclusion; Acknowledgments. 2 Methodological Requirements for Lipidomics Research1. Sample Preparation and Lipid Extraction; 2. Sample Preparation; 3. Lipid Extraction; 4. Sample Preparation and Lipid Extraction of Subcellular Organelles; 5. Chromatography-Coupled Mass Spectrometry of Lipids; 6. Shotgun Lipidomics -- Direct Infusion-Based Mass Spectrometry of Lipids; 7. Postacquisition Data Processing and Bioinformatics; 8. Conclusion; Acknowledgments; 3 Biological Methods for Metabolic Research; 1. Gene Overexpression as a Tool for Metabolomic Studies; 1.1. Gene Transfection: Plasmid Vectors. 1.2. Gene Transduction: Viral Vectors2. Gene Silencing as a Tool for Metabolomic Studies; 3. Validation of Gene Overexpression or Silencing by mRNA; 3.1. Gene Expression Analysis; 3.1.1. Reverse Transcriptase Polymerase Chain Reaction; 3.1.2. Real-Time Polymerase Chain Reaction; 4. Validation of Gene Overexpression or Silencing by Protein Level Analysis and Enzyme Assays; 4.1. Western Blotting; 4.1.1. Blotting; 4.1.2. Immunodetection; 4.2. Enzyme Assays; 4.2.1. Protocols for Analysis of Choline Kinase- Activity; 4.2.2. Protocol for Analysis of Phospholipase D Activity. 5. Enzymatic Assays as a Tool of Phospholipid Metabolism Analysis: Effects of Choline Kinase- Inhibition on the Balance of Phospholipid Metabolites5.1. Protocol for Lipid Analysis; 5.2. Protocol for Analysis of Phosphatidylinositol-3-Kinase Activity; 6. Phospholipid Profile Analysis by Mass Spectrometry; 6.1. Phospholipid Extraction; 6.2. Analytical Technique for Phospholipids: Mass Spectrometry; Section 2 Metabolomic Mass Spectrometry: Experimental Techniques and Bioinformatics. 4 Considerations in Sample Preparation, Collection, and Extraction Approaches Applied in Microbial, Plant, and Mammalian Metabolic Profiling1. Considerations in the Culturing, Collection, Preparation, and Extraction of Microbial Samples; 1.1. Sample Types; 1.2. Culture Methods; 1.2.1. Batch Growth; 1.2.2. Continuous Culture; 1.2.3. Selection of Media; 1.3. Experimental Considerations in the Collection and Preparation of Samples; 1.3.1. Quenching of Cultures; 1.3.2. Assessment of Leakage; 1.3.3. Extraction of Intracellular Metabolites. |
| 요약 | Methodologies for Metabolomics provides comprehensive descriptions of the newest methodological strategies and techniques in metabolomic research. |
| 일반주제명 | Mass spectrometry --Methods. Metabolism --Disorders --Diagnosis. Metabolites. Metabolomics --methods. Metabolome. Chemistry Techniques, Analytical. Biochemistry. Computational Biology. Analytical, Diagnostic and Therapeutic Techniques and Equipment. Metabolism. Biology. Disease. Phenomena and Processes. Biological Science Disciplines. Chemistry. Natural Science Disciplines. Disciplines and Occupations. Diagnosis. Mass Spectrometry. Metabolic Diseases. SCIENCE --Chemistry --Analytic. |
| 언어 | English. |
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