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BHLF1, a Lytic-Cycle Gene Encoding a Long Non-Coding RNA Contributes to Epstein-Barr Virus Latency
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | BHLF1, a Lytic-Cycle Gene Encoding a Long Non-Coding RNA Contributes to Epstein-Barr Virus Latency. |
| 개인저자 | Yetming, Kristen Dominique. |
| 단체저자명 | The Pennsylvania State University. |
| 발행사항 | [S.l.]: The Pennsylvania State University., 2017. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2017. |
| 형태사항 | 199 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-01B. Dissertation Abstract International |
| ISBN | 9781392335680 |
| 학위논문주기 | Thesis (Ph.D.)--The Pennsylvania State University, 2017. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-01, Section: B.
Publisher info.: Dissertation/Thesis. Advisor: Sample, Jeffery T. |
| 요약 | Epstein-Barr virus (EBV) is a lymphotropic, gammaherpesvirus which efficiently establishes a persistent, lifelong infection in humans. As is common with other herpesviruses, EBV has both replicative (lytic) and latent cycles of infection. EBV latency, in which there is no virus production, can be subdivided into several distinct latency programs which are characterized by differential expression patterns of the viral latency-associated proteins: EBNA1, -2, -3A, -3B, -3C, -LP and LMP1, -2A, -2B. The major long-term reservoir of EBV is the memory B cell, and as latently-infected B cells progress from an initial state of EBV-driven cell proliferation to a state of long-term viral latency within the memory B-cell pool, there is a restriction in the expression of the viral latency proteins, due to the epigenetic silencing of the EBNA promoters Wp and Cp. This restriction in latency is essential for the persistence of EBV infection as cytotoxic T lymphocytes can remove infected B cells that continue to express several of the latencyassociated proteins.Historically, it has been thought that latency-associated EBV gene products only contribute to the latent cycle, and lytic-cycle genes only function during lytic infection. However, it has recently been found that there is a subset of "lytic" genes that are expressed upon the initiation of latency, one of which is BHLF1. This, along with several other lines of evidence, suggests that BHLF1 may have a latency-associated function. Thus, the goal of the work presented in this dissertation was to elucidate whether BHLF1 has a role in the establishment or maintenance of EBV latency. Using recombinant EBV (rEBV), we observed that infection of an EBV-negative cell line, BL2, with a wild-type (WT) rEBV is capable of sustaining latency III, whereas infection with mutant rEBVs, in which BHLF1 has been deleted, results in a transition from latency III to latency I within 3 months post-infection at both the protein and mRNA levels. Disruption of BHLF1 did not significantly influence the expression of other genes near the locus |
| 일반주제명 | Molecular biology. Microbiology. Virology. |
| 언어 | 영어 |
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