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The Novel Long Noncoding RNA Pnky Regulates Neurogenesis and Neural Stem Cell Maintenance In Vivo
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | The Novel Long Noncoding RNA Pnky Regulates Neurogenesis and Neural Stem Cell Maintenance In Vivo. |
| 개인저자 | Andersen, Rebecca Ellen. |
| 단체저자명 | University of California, San Francisco. Developmental and Stem Cell Biology. |
| 발행사항 | [S.l.]: University of California, San Francisco., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 186 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-04B. Dissertation Abstract International |
| ISBN | 9781085649759 |
| 학위논문주기 | Thesis (Ph.D.)--University of California, San Francisco, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Advisor: Lim, Daniel A. |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | While it is now appreciated that certain long noncoding RNAs (lncRNAs) have important functions in cell biology, relatively few have been shown to regulate development in vivo, particularly with genetic strategies that establish cis versus trans mechanisms. We have identified Pinky (Pnky) as a lncRNA that regulates neurogenesis in the embryonic and postnatal mouse brain. Pnky is a nuclear-enriched lncRNA that is divergent to the neighboring proneural transcription factor Pou3f2. In postnatal neural stem cells (NSCs) from the ventricular-subventricular zone (V-SVZ), Pnky knockdown promotes neuronal lineage commitment and expands the transit amplifying cell population, increasing neuron production several-fold. In the embryonic cortex, Pnky knockdown increases neuronal differentiation and depletes the NSC population prematurely. Furthermore, genetic deletion of Pnky results in the aberrant production of neuronal masses along the postnatal V-SVZ. In the developing cortex, Pnky regulates the production of projection neurons from NSCs in a cell-autonomous manner, and loss of Pnky alters postnatal cortical lamination. Surprisingly, Pou3f2 expression is not disrupted by deletion of the entire Pnky gene. Moreover, expression of Pnky from a bacterial artificial chromosome (BAC) transgene rescues the differential gene expression and increased neurogenesis of Pnky-knockout NSCs, as well as the developmental phenotypes of Pnky-deletion in vivo. Thus, despite being transcribed divergently from a key developmental transcription factor, the lncRNA Pnky regulates neural development in trans. |
| 일반주제명 | Developmental biology. Neurosciences. Molecular biology. Cellular biology. Genetics. |
| 언어 | 영어 |
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: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
