상세정보
Export to Refworks부가기능
Yeast Adaptation and Survival under Acute Exposure to Lethal Ethanol Stress
상세 프로파일
| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Yeast Adaptation and Survival under Acute Exposure to Lethal Ethanol Stress. |
| 개인저자 | Yang, Jamie S. |
| 단체저자명 | Columbia University. Cellular, Molecular and Biomedical Studies. |
| 발행사항 | [S.l.]: Columbia University., 2020. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2020. |
| 형태사항 | 151 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-02B. Dissertation Abstract International |
| ISBN | 9781085608626 |
| 학위논문주기 | Thesis (Ph.D.)--Columbia University, 2020. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-02, Section: B.
Advisor: Tavazoie, Saeed. |
| 이용제한사항 | This item must not be sold to any third party vendors.This item must not be added to any third party search indexes. |
| 요약 | The ability to respond to stress is universal in all domains of life. Failure to properly execute the stress response compromises the fitness of the organism. Several key stress pathways are conserved from unicellular organisms to higher eukaryotes, so knowledge of how these pathways operate in model organisms is crucial for understanding stress-related diseases and aging in humans. The mechanisms of stress tolerance have been well-studied in the budding yeast Saccharomyces cerevisiae. Yeast respond to diverse stresses by initiating both general and stress-specific responses that generally protect the cells during and after the stress exposure. While previous work has revealed mechanistic insights on adaptation and survival under mild and long-term exposure to stress, how they cope with acute exposure to lethal stress is not well understood.Here, we combined transcriptional profiling, fitness profiling, and laboratory evolution to investigate how S. cerevisiae survive acute exposure to lethal ethanol stress. By using high throughput methods such as RNA-seq and barcode sequencing of the pooled yeast deletion library, we were able to discover and characterize both existing and novel pathways that yeast utilize to adapt to and survive ethanol stress. We found both ethanol-specific and as well general stress response mechanisms. We were also able to evolve a strain of ethanol under lethal ethanol stress to exhibit a survival of at least an order of magnitude greater than the parental wild-type strain. Additionally, this evolved strain exhibited cross protection to other stresses without compromising bulk growth rate. We found that this strain adapted its global expression levels to a post-stress state, making it more robust to various stresses even under optimal growth conditions. |
| 일반주제명 | Biology. Genetics. Microbiology. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
