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Applications of SAMDI Mass Spectrometry for High-Throughput Reaction Monitoring on Peptide Arrays
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Applications of SAMDI Mass Spectrometry for High-Throughput Reaction Monitoring on Peptide Arrays. |
| 개인저자 | Szymczak, Lindsey C. |
| 단체저자명 | Northwestern University. Chemistry. |
| 발행사항 | [S.l.]: Northwestern University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 202 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-04B. Dissertation Abstract International |
| ISBN | 9781088315569 |
| 학위논문주기 | Thesis (Ph.D.)--Northwestern University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Advisor: Mrksich, Milan. |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | This work combines the use of high-throughput mass spectrometry with peptide arrays for to monitor reactions on peptides. The Mrksich lab introduced a high-throughput, label-free, biochemical assay that relies on self-assembled monolayers on gold and matrix-assisted laser desorption/ionization mass spectrometry, termed SAMDI-MS. This dissertation introduces new applications of SAMDI-MS and peptide arrays and illustrates the many advantages of this technique for studying reactions on peptides.Chapter 2 describes the use of SAMDI-MS and peptide arrays for the identification of new sequence-selective reactions of peptides. Selective reactions on peptides and proteins are important for studying protein function and preparing protein-based materials. Using this technique, we discover and characterize the sequence-selective acetylation of peptides containing histidine and tyrosine by acetic anhydride and demonstrate the advantages of using peptide arrays with a label- free analysis method to discover peptide-modifying reactions.We then illustrate the use of SAMDI-MS and phosphorylated peptide arrays to gain a systems- level understanding of phosphatase activity in cell lysates. Phosphorylation is the most prominent post-translational modification on proteins. While phosphorylation plays important roles in many cellular processes, understanding of the regulation of phosphorylation is limited. Kinases and phosphatases work cooperatively to regulate phosphorylation |
| 일반주제명 | Chemistry. Biochemistry. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
