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Characterization of a Plasmid Encoded Sigma Factor Endogenous to Bacillus Subtilis
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Characterization of a Plasmid Encoded Sigma Factor Endogenous to Bacillus Subtilis. |
| 개인저자 | Burton, Aisha Tafa. |
| 단체저자명 | Indiana University. Biology. |
| 발행사항 | [S.l.]: Indiana University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 160 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-04B. Dissertation Abstract International |
| ISBN | 9781088355978 |
| 학위논문주기 | Thesis (Ph.D.)--Indiana University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Advisor: Kearns, Daniel B. |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | Bacteria use sigma factors to regulate and control gene expression. Extra cytoplasmic function sigma factors are activated during times of stress to enhance the survival of the cell. Here we show that pBS32, a large, low copy number plasmid present in the ancestral strain of Bacillus subtilis, encodes an extra cytoplasmic function sigma factor, SigN, that mediates cell death after DNA damage. Using molecular biology, biochemistry, and genetics, SigN was characterized. We show that SigN interacts with RNAP to initiate transcription, is repressed by LexA, autoactivates, and contains a regulon on pBS32. The mechanism of death remains unknown, but we know that the global transcriptional repressor, AbrB, is needed for death to occur. With these new findings, we have increased the knowledge base of pBS32 and expanded what we currently know about extra cytoplasmic function sigma factors. |
| 일반주제명 | Microbiology. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
