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Experimental Characteristics of Diffuse X-Ray Scattering in Lysozyme Crystals
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Experimental Characteristics of Diffuse X-Ray Scattering in Lysozyme Crystals. |
| 개인저자 | Pillar, Veronica Drabek. |
| 단체저자명 | Cornell University. Physics. |
| 발행사항 | [S.l.]: Cornell University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 143 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-04B. Dissertation Abstract International |
| ISBN | 9781088379707 |
| 학위논문주기 | Thesis (Ph.D.)--Cornell University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Advisor: Gruner, Sol. |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | X-ray crystallography, a popular and powerful technique for determining the atomic structures of proteins and other macromolecules, is traditionally limited to reporting static structures, whereas proteins in vivo are constantly shifting between conformations. Such conformational dynamics give rise to structured diffuse scattering, which is observable in protein crystallography experiments. Because the measurement of diffuse scattering involves numerous experimental challenges beyond those inherent to traditional crystallography, some basic questions about the behavior of diffuse intensities remain unanswered: how reproducible is the diffuse intensity between ostensibly identical crystals? What happens to diffuse intensity when the protein binds to an inhibitor molecule, when the crystal is cryocooled, or when it suffers radiation damage? Here, we present the results of diffuse scattering experiments on 23 different lysozyme crystals under a variety of experimental conditions. It is shown that diffuse intensity is highly consistent between ostensibly identical room temperature crystals. Binding to an inhibitor reduces the diffuse intensity non-uniformly, suggesting a change in the molecular motions that produce diffuse scattering. Cryocooling drastically changes the diffuse intensity pattern, indicating that cryocooled crystals are poor representatives of the correlated displacements in room temperature crystals. Radiation dose effects in a single image's diffuse scattering are also discussed. |
| 일반주제명 | Physics. Biophysics. |
| 언어 | 영어 |
| 바로가기 | 
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