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Copper Homeostasis Genes in Fibroporia radiculosa and How They Relate to Copper-tolerance
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Copper Homeostasis Genes in Fibroporia radiculosa and How They Relate to Copper-tolerance. |
| 개인저자 | Ohno, Katie M. |
| 단체저자명 | The University of Wisconsin - Madison. Forestry. |
| 발행사항 | [S.l.]: The University of Wisconsin - Madison., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 120 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-02B. Dissertation Abstract International |
| ISBN | 9781085764247 |
| 학위논문주기 | Thesis (Ph.D.)--The University of Wisconsin - Madison, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-02, Section: B.
Advisor: Stanosz, Glen R. |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | Brown-rot basidiomycete decay fungi are the greatest threat to wood products in service, because they can lessen the efficacy of copper-containing wood preservative formulations. It is critical to investigate the mechanism of copper-tolerance to prevent copper-treated wood deterioration by these organisms. The goal of this research was to contribute to the understanding of this mechanism in Fibroporia radiculosa. Copper removal during decay of copper-treated southern pine (Pinus spp.) by two isolates of F. radiculosa and copper-sensitive Gloeophyllum trabeum was quantified. Expression of a cytochrome p450 monooxygenase (p450) and a superoxide dismutase (SOD), thought to be involved in inducing intracellular responses to unfavorable environments, were evaluated during fungal decay of non-treated and copper-treated southern pine. Additionally, expression of a copper homeostasis CutC protein (CutC) and three copper-transporting ATPase pumps (ATPase1, ATPase2, and ATPase3), thought to be involved in overcoming toxic copper environments were evaluated. Both F. radiculosa isolates were able to decay copper-treated wood, and one isolate caused more weight loss on copper treated wood than on non-treated wood. This isolate also removed substantial amounts of copper from the test specimens. Expression of the p450 and the SOD enzymes was down-regulated or not detected initially, but SOD expression was strongly upregulated on copper-treated wood for one F. radiculosa isolate at 4 weeks. CutC expression was not detected for G. trabeum and one F. radiculosa isolate, but was down-regulated more strongly on copper-treated wood than untreated wood for the other F. radiculosa isolate. G. trabeum strongly upregulated ATPase1 on copper-treated wood but the two copper-tolerant fungi did not. G. trabeum and one F. radiculosa isolate initially upregulated ATPase2 on copper-treated wood but subsequently expression was either not detected or was similar to untreated wood. The other F. radiculosa isolate consistently down-regulated ATPase2. ATPase3 expression by G. trabeum was not detected, but was upregulated by both F. radiculosa isolates. It can be suggested that the two F. radiculosa isolates utilized different mechanisms from each other, and from G. trabeum, when decaying copper-treated wood. Future research is needed to clarify how copper-tolerant brown-rot fungi manage toxic copper environments. |
| 일반주제명 | Forestry. Microbiology. Molecular biology. |
| 언어 | 영어 |
| 바로가기 | 
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