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Arenavirus-Host Interactions: Roles of Viral Glycoprotein and Nucleoprotein in Mediating Cell Entry and Host Immune Suppression
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Arenavirus-Host Interactions: Roles of Viral Glycoprotein and Nucleoprotein in Mediating Cell Entry and Host Immune Suppression. |
| 개인저자 | Shao, Junjie. |
| 단체저자명 | University of Minnesota. Veterinary Medicine. |
| 발행사항 | [S.l.]: University of Minnesota., 2018. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2018. |
| 형태사항 | 119 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-04B. Dissertation Abstract International |
| ISBN | 9781085754903 |
| 학위논문주기 | Thesis (Ph.D.)--University of Minnesota, 2018. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Advisor: Liang, Yuying |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | Several mammalian arenaviruses can infect humans and non-human primates and can cause severe and deadly hemorrhagic fever diseases. There are currently no FDA-approved vaccines available for the prevention of infection by any of these pathogenic arenaviruses and treatment options for the infections are extremely limited. Arenavirus is an enveloped, bi-segmented, single-stranded RNA virus. Its genome encodes four viral proteins: the RNA-dependent RNA polymerase L, the nucleoprotein NP, the glycoprotein complex GPC, and the matrix protein Z. Arenaviral GPC plays critical roles in the first step of virus infection (i.e, cell entry), which involves receptor recognition and virus-host membrane fusion activity. Arenaviral NP is a multifunctional protein, which is involved in the formation of the viral ribonucleoprotein (RNP) complex needed for viral genome replication and transcription, and in host immunosuppression. Arenaviral L protein, together with NP, is responsible for genome replication and transcription. Arenaviral Z protein is the driving force of virion budding from the membrane of the infected cells and is also involved in mediating immune suppression. My study focused on the roles of arenaviral GPC and NP in mediating cell entry and host immune suppression. Arenaviral GPC is synthesized as a single polypeptide and is post-translationally processed by the cellular signal peptidase and S1P cellular protease into stable signal peptide (SSP), the receptor-binding GP1 and transmembrane GP2 subunits. My thesis focused on characterizing (1) the role of the glycoprotein SSP in mediating entry of the Pichinde virus (PICV) into cells in culture and its role in disease pathogenesis in guinea pigs, (2) the role of several conserved amino acids residues in the glycoprotein GP2 C-terminal domain (CTD) of PICV in mediating virus entry in vitro, and (3) the role of NP in mediating immune suppression via the PACT-RIG-I innate immune pathway. |
| 일반주제명 | Microbiology. Epidemiology. Immunology. Virology. |
| 언어 | 영어 |
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