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Revealing Hidden Biology by Identifying Ligands for the ykkC Orphan Riboswitches
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Revealing Hidden Biology by Identifying Ligands for the ykkC Orphan Riboswitches. |
| 개인저자 | Sherlock, Madeline Elizabeth. |
| 단체저자명 | Yale University. |
| 발행사항 | [S.l.]: Yale University., 2018. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2018. |
| 형태사항 | 299 p. |
| 기본자료 저록 | Dissertation Abstracts International 79-12B(E). Dissertation Abstract International |
| ISBN | 9780438194779 |
| 학위논문주기 | Thesis (Ph.D.)--Yale University, 2018. |
| 일반주기 |
Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B.
Adviser: Ronald R. Breaker. |
| 요약 | Riboswitches are non-coding RNAs that bind a small molecule or ion ligand to regulate gene expression. The cognate ligands have been identified for dozens of riboswitch classes, but the ligand identities are unknown for many promising structured |
| 요약 | My colleagues found that guanidine induces expression of a riboswitch- lacZ reporter gene fusion in a large screen of various growth conditions. They collected additional evidence implicating guanidine as the elusive ligand for ykkC riboswitches |
| 요약 | I subsequently determined that representatives of two other RNA motifs, called mini-ykkC and ykkC-III, also bind guanidine in vitro. All three guanidine-binding RNA motifs have similar downstream gene associations, and therefore these three dist |
| 요약 | While the majority of ykkC RNAs selectively bind guanidine, I found that the remaining 30% of ykkC RNAs do not bind guanidine and carry nucleotide changes in their binding pockets to allow recognition of a different ligand. I analyzed the consen |
| 요약 | I discovered that the first variant class selectively binds guanosine tetraphosphate (ppGpp), a bacterial alarmone derived from the ribonucleotide GTP. The ppGpp riboswitch class primarily regulates transcription of genes involved in branched-ch |
| 일반주제명 | Biochemistry. Microbiology. Molecular biology. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
