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Rna Kissing-Loop Interactions Supported By Bifacial Peptide Nucleic Acid
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Rna Kissing-Loop Interactions Supported By Bifacial Peptide Nucleic Acid. |
| 개인저자 | DeSantis, Christopher Amedeo. |
| 단체저자명 | The Ohio State University. Chemistry. |
| 발행사항 | [S.l.]: The Ohio State University., 2017. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2017. |
| 형태사항 | 291 p. |
| 기본자료 저록 | Dissertation Abstracts International 80-01B(E). Dissertation Abstract International |
| ISBN | 9780438309654 |
| 학위논문주기 | Thesis (Ph.D.)--The Ohio State University, 2017. |
| 일반주기 |
Source: Dissertation Abstracts International, Volume: 80-01(E), Section: B.
Adviser: Dennis Bong,Dr. |
| 요약 | The dissertation to follow is an account of research efforts to utilize bifacial peptide nucleic acids (bPNA) triplexes to support kissing-loop complexes. A kissing-loop complex is composed of two nucleic acid stem-loops hybridized via Watson-Cr |
| 요약 | In the first part if this work, kissing-loop and Rop complex formation was analyzed in titration binding assays and native gel electrophoresis. tRNA duplex-triplex kissing complexes show a 5-fold decrease in affinity while omission of bPNA decre |
| 요약 | In the latter part of this work, fluorescently-labeled bPNA were used to monitor kissing-loop formation in vitro. Triplex-triplex kissing-loop formation was monitored by FRET using Cy3- and Cy5-labeled bPNA triplexes. Competitive displacement of |
| 요약 | In summary, we have shown that hairpins mutated with a bPNA triplex in the stem region are able to form kissing-loop complexes. Furthermore, proteins which interact with kissing-loops are still able to bind complexes with the triplex mutation. U |
| 일반주제명 | Chemistry. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
