자료유형 | 학위논문 |
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서명/저자사항 | Advancements in Single Molecule Localization Microscopy for Improved Multichannel, 3-Dimensional, and Structurally-Dense Imaging. |
개인저자 | Gagnon, Lauren Ann. |
단체저자명 | University of Washington. Chemistry. |
발행사항 | [S.l.]: University of Washington., 2019. |
발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
형태사항 | 112 p. |
기본자료 저록 | Dissertations Abstracts International 81-04B. Dissertation Abstract International |
ISBN | 9781088325438 |
학위논문주기 | Thesis (Ph.D.)--University of Washington, 2019. |
일반주기 |
Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Includes supplementary digital materials. Advisor: Vaughan, Joshua C. |
이용제한사항 | This item must not be sold to any third party vendors.This item must not be added to any third party search indexes. |
요약 | Super-resolution fluorescence microscopy now enables cellular detail to be probed at the nanometer scale using far-field optics with widely available instrumentation. However, single molecule localization microscopy (SMLM) techniques that rely on the stochastic on-off switching of individual fluorophores to achieve higher resolution face significant drawbacks when trying to achieve multicolor, 3-D, and structurally-dense imaging. This is due to limitations in the photoswitchable fluorophores currently available. Here, I provide a detailed discussion of photoswitchable fluorophores for SMLM and the challenges they create. I also present a new method, prSTORM, which is capable of using a single fluorophore to image all channels and achieve 3-D imaging at extended depths through the use of DNA barcodes. Additionally, I propose how this methodology can be extended to high structural density imaging without compromising resolution. |
일반주제명 | Chemistry. Microscopy. |
언어 | 영어 |
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