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HIV-1 Engages a Dynein-Dynactin-BICD2 Complex for Infection and Transport to the Nucleus
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | HIV-1 Engages a Dynein-Dynactin-BICD2 Complex for Infection and Transport to the Nucleus. |
| 개인저자 | Carnes, Stephanie K. |
| 단체저자명 | Vanderbilt University. Microbiology and Immunology. |
| 발행사항 | [S.l.]: Vanderbilt University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 158 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-04B. Dissertation Abstract International |
| ISBN | 9781088318706 |
| 학위논문주기 | Thesis (Ph.D.)--Vanderbilt University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Advisor: Aiken, Christopher. |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | HIV-1 infection depends on efficient intracytoplasmic transport of the incoming viral core to the target cell nucleus. Evidence suggests that this movement is facilitated by the microtubule motor dynein, a large multi-protein complex that interacts with dynactin and cargo-specific adaptor proteins for retrograde movement via microtubules. Dynein adaptor proteins are necessary for activating dynein movement and for linking specific cargoes to dynein. I hypothesized that HIV-1 engages the dynein motor complex via an adaptor for intracellular transport. Here, I show that siRNA depletion of the dynein heavy chain, components of the dynactin complex, and the dynein adaptor BICD2, reduced cell permissiveness to HIV-1 infection. Cell depletion of dynein heavy chain and BICD2 resulted in impaired HIV-1 DNA accumulation in the nucleus and decreased retrograde movement of the virus. Biochemical studies revealed that dynein components and BICD2 associate with capsid-like assemblies of the HIV-1 CA protein in cell extracts and that purified recombinant BICD2 binds to CA assemblies in vitro. Association of dynein with CA assemblies was reduced upon immunodepletion of BICD2 from cell extracts. I conclude that BICD2 is a capsid-associated dynein adaptor utilized by HIV-1 for transport to the nucleus. |
| 일반주제명 | Virology. Cellular biology. Biochemistry. |
| 언어 | 영어 |
| 바로가기 | 
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