상세정보
Export to Refworks부가기능
Cancer Dynamics under a Chemotherapeutic Stress Gradient Using a Microfluidic in vitro Tumor Environment
상세 프로파일
| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Cancer Dynamics under a Chemotherapeutic Stress Gradient Using a Microfluidic in vitro Tumor Environment. |
| 개인저자 | Lin, Ke-Chih. |
| 단체저자명 | Princeton University. Electrical Engineering. |
| 발행사항 | [S.l.]: Princeton University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 132 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-03B. Dissertation Abstract International |
| ISBN | 9781085635882 |
| 학위논문주기 | Thesis (Ph.D.)--Princeton University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-03, Section: B.
Advisor: Sturm, James C. |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | In this dissertation, we use the microfluidic cancer-on-chip system we have developed to explore cancer population dynamics and how cancer acquires drug resistance. The microfluidic cell culture device, the "evolution accelerator" (EA), generates an in vitro landscape of stress heterogeneity across a tumor population. The system allows for high-magnification real-time observations of different cancer cell lines and downstream analysis of cell phenotype as a function of position on the stress landscape. With the EA technology, we investigate the adaptation and evolution dynamics in prostate cancer cell metapopulations under a stress landscape of a chemotherapeutic drug (docetaxel). High-resolution time-lapse scanning provides abundant information about the change in cell morphology, population dynamics, cell motility and cell migration over time on a cellular level.We further implement this technology to study quantitatively the emergence of polyploid, mesenchymal and stem-like cancer cells in the context of complex heterogeneous yet controllable in vitro environments with a spatially-varying drug concentration. Within our microfluidic stress landscape, we observe: (1) a previously-unobserved surprisingly large number of polyploid giant cancer cells (PGCCs) which emerged in a highly stressful region in response to chemotherapy |
| 일반주제명 | Biomedical engineering. Biophysics. Physiology. Medicine. Epidemiology. Public health. Oncology. Health sciences. Pathology. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
