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Dynamics and Assembly of Divisome and Cell Wall Synthesis Proteins in the Prolate Ellipsoid-shaped, "Superbug" Pathogen, Streptococcus pneumoniae
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Dynamics and Assembly of Divisome and Cell Wall Synthesis Proteins in the Prolate Ellipsoid-shaped, "Superbug" Pathogen, Streptococcus pneumoniae. |
| 개인저자 | Perez, Amilcar J. |
| 단체저자명 | Indiana University. Biology. |
| 발행사항 | [S.l.]: Indiana University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 407 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-05B. Dissertation Abstract International |
| ISBN | 9781088390900 |
| 학위논문주기 | Thesis (Ph.D.)--Indiana University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-05, Section: B.
Advisor: Winkler, Malcolm E. |
| 이용제한사항 | This item must not be sold to any third party vendors.This item must not be added to any third party search indexes. |
| 요약 | Bacterial cell shape and cell division are orchestrated by essential protein complexes that dynamically synthesize the cell wall (also termed peptidoglycan or PG). Studies of dynamic mechanisms that synthesize PG have been largely confined to model rod-shaped bacteria. Yet, a diverse array of evolved cell shapes exist that play key roles in growth, survival, and pathogenesis. Therefore, there is a major gap in knowledge in understanding fundamental mechanisms that result in different cell shapes and modes of division and PG synthesis. My research focuses on the dynamic protein complexes that determine cell shape and cell division in the prolate ellipsoid-shaped (ovococcal) Gram-positive bacterial pathogen, Streptococcus pneumoniae. I combine genetic and chemical perturbations with fluorescence microscopy to study the organization and dynamics of the cytoplasmic filament systems (FtsZ/FtsA/EzrA) and their relationship to the dynamics of the cell wall synthases involved in septum formation (bPBP2x:FtsW) and cellular elongation (bPBP2b:RodA).This work elucidates several key differences of cell biology that deviate from the paradigm established in model rod-shaped bacteria indicating that the movement of both these enzyme pairs is reflective of PG synthesis. Lastly, I discover the essential role of EzrA in division site selection in S. pneumoniae using classical genetic approaches combined with fluorescence microscopy. By studying the dynamic assembly and synthesis of the cell wall this work may provide new ways to target antibiotic resistant bacteria. |
| 일반주제명 | Microbiology. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
