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The Encapsulation of an E. coli TXTL in Cell-sized Compartments Towards Prototyping Synthetic Cells
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | The Encapsulation of an E. coli TXTL in Cell-sized Compartments Towards Prototyping Synthetic Cells. |
| 개인저자 | Garamella, Jonathan T. |
| 단체저자명 | University of Minnesota. Physics. |
| 발행사항 | [S.l.]: University of Minnesota., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 127 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-04B. Dissertation Abstract International |
| ISBN | 9781085772792 |
| 학위논문주기 | Thesis (Ph.D.)--University of Minnesota, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Advisor: Noireaux, Vincent. |
| 이용제한사항 | This item must not be sold to any third party vendors.This item must not be added to any third party search indexes. |
| 요약 | The bottom-up assembly of synthetic cell systems capable of recapitulating biological functions has become a means to understand living matter through construction. Cell-free protein synthesis platforms, which allow for the rapid prototyping of biological systems by reducing the design-build-test cycle relative to in vivo experiments, have emerged as a tool to achieve this goal. In this dissertation, I report on the most recent iteration of an all E. coli TXTL and its use towards the realization of a bottom-up synthetic cell. TXTL offers robust protein synthesis with access to the full complement of regulatory parts available in E. coli. I detail efforts to encapsulate TXTL into cell-sized liposomes, providing researchers a platform to carry out complex reactions in containers in which the local environment and membrane composition can be altered. While a functional E. coli divisome was not reconstructed, the FtsZ and MreB family of proteins were expressed in liposomes, with MreB showing significant deformation. Finally, I developed synthetic cell prototypes programmed to be mechanosensitive, coupling this function to create multiple synthetic cell prototypes that are biosensing or adaptive based on the local environment. |
| 일반주제명 | Physics. Biophysics. |
| 언어 | 영어 |
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