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A Strategy for Combinatorial Cavity Design in De Novo Proteins
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | A Strategy for Combinatorial Cavity Design in De Novo Proteins. |
| 개인저자 | Karas, Christina E. |
| 단체저자명 | Princeton University. Molecular Biology. |
| 발행사항 | [S.l.]: Princeton University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 175 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-06B. Dissertation Abstract International |
| ISBN | 9781392880005 |
| 학위논문주기 | Thesis (Ph.D.)--Princeton University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-06, Section: B.
Advisor: Hecht, Michael H. |
| 이용제한사항 | This item must not be sold to any third party vendors. |
| 요약 | Protein sequence space is vast |
| 요약 | 6 unique proteins, assembled from degenerate oligonucleotides. The third and fourth parts of this work cover the screening effort for a range of activities, both in vitro and in vivo. I found that this collection binds heme readily, leading to abundant peroxidase activity. Hits for lipase and phosphatase activity were also detected.This thesis details the development of a new strategy for creating de novo sequences geared toward function rather than structure. Following my work, these library design principles are being applied to SynF4, a de novo enterobactin esterase, whose structure was recently solved. By diversifying the cavity of SynF4, we hope to create a new family of de novo enzymes. Altogether, this approach represents a step towards creating artificial proteomes capable of carrying out essential biological roles. |
| 일반주제명 | Molecular biology. Chemistry. Bioengineering. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
