자료유형 | 학위논문 |
---|---|
서명/저자사항 | A Strategy for Combinatorial Cavity Design in De Novo Proteins. |
개인저자 | Karas, Christina E. |
단체저자명 | Princeton University. Molecular Biology. |
발행사항 | [S.l.]: Princeton University., 2019. |
발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
형태사항 | 175 p. |
기본자료 저록 | Dissertations Abstracts International 81-06B. Dissertation Abstract International |
ISBN | 9781392880005 |
학위논문주기 | Thesis (Ph.D.)--Princeton University, 2019. |
일반주기 |
Source: Dissertations Abstracts International, Volume: 81-06, Section: B.
Advisor: Hecht, Michael H. |
이용제한사항 | This item must not be sold to any third party vendors. |
요약 | Protein sequence space is vast |
요약 | 6 unique proteins, assembled from degenerate oligonucleotides. The third and fourth parts of this work cover the screening effort for a range of activities, both in vitro and in vivo. I found that this collection binds heme readily, leading to abundant peroxidase activity. Hits for lipase and phosphatase activity were also detected.This thesis details the development of a new strategy for creating de novo sequences geared toward function rather than structure. Following my work, these library design principles are being applied to SynF4, a de novo enterobactin esterase, whose structure was recently solved. By diversifying the cavity of SynF4, we hope to create a new family of de novo enzymes. Altogether, this approach represents a step towards creating artificial proteomes capable of carrying out essential biological roles. |
일반주제명 | Molecular biology. Chemistry. Bioengineering. |
언어 | 영어 |
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