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Regulation of Microtubule Dynamics via Abl2 Binding
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| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | Regulation of Microtubule Dynamics via Abl2 Binding. |
| 개인저자 | Hu, Yuhan. |
| 단체저자명 | Yale University. Cell Biology. |
| 발행사항 | [S.l.]: Yale University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 114 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-03B. Dissertation Abstract International |
| ISBN | 9781085787680 |
| 학위논문주기 | Thesis (Ph.D.)--Yale University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-03, Section: B.
Advisor: Koleske, Anthony J. |
| 이용제한사항 | This item must not be sold to any third party vendors.This item must not be added to any third party search indexes. |
| 요약 | Abl family kinases are believed to play as central signaling nodes to coordinate microtubule dynamics by transducing signals from upstream guidance receptors to downstream microtubule-associated proteins. Here, I report a novel microtubule dynamics regulation mechanism via direct binding of Abl2. In this study, I discovered that Abl2 directly binds microtubules and regulate microtubule dynamics. In a purified recombinant system, I identified a central proline-rich region inside Abl2 that is sufficient and necessary to bind to microtubules, and the interaction between Abl2 and microtubules is electrostatically mediated by the COOH-terminal acidic tubulin tail. Using in vitro single-filament microscopy, I found that Abl2 stably bound onto microtubule lattice and regulated microtubule dynamics by increasing elongation rate, reducing shortening rate, and decreasing catastrophe frequency. In cells, when Abl2 was knocked out microtubule growth rate was significant reduced and re-expressing Abl2 successfully rescued the microtubule growth defect. These and other data suggest a model where Abl2 act as a microtubule regulator at the cell periphery. |
| 일반주제명 | Biology. Biochemistry. Cellular biology. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
