상세정보
Export to Refworks부가기능
The Role of Protein-Nucleic Acid Interactions in the Function and Fidelity of DNA Polymerase 棺 and CRISPR Cas9 Monitored by NMR
상세 프로파일
| 자료유형 | 학위논문 |
|---|---|
| 서명/저자사항 | The Role of Protein-Nucleic Acid Interactions in the Function and Fidelity of DNA Polymerase 棺 and CRISPR Cas9 Monitored by NMR. |
| 개인저자 | East, Kyle William. |
| 단체저자명 | Yale University. Chemistry. |
| 발행사항 | [S.l.]: Yale University., 2019. |
| 발행사항 | Ann Arbor: ProQuest Dissertations & Theses, 2019. |
| 형태사항 | 148 p. |
| 기본자료 저록 | Dissertations Abstracts International 81-03B. Dissertation Abstract International |
| ISBN | 9781085777407 |
| 학위논문주기 | Thesis (Ph.D.)--Yale University, 2019. |
| 일반주기 |
Source: Dissertations Abstracts International, Volume: 81-03, Section: B.
Advisor: Loria, J. Patrick. |
| 이용제한사항 | This item must not be sold to any third party vendors.This item must not be added to any third party search indexes. |
| 요약 | Protein-nucleic acid interactions are one of the most important processes required for life on earth. These interactions make up the core of the central dogma of molecular biology first published by Francis Crick in 1958. For over six decades, the interactions of proteins with DNA and RNA have been heavily studied in order to better understand how the proteome interacts with the genome and nucleic acid polymers. The complexity of these interactions is best highlighted by the thousands of proteins that interact with genomic DNA and various RNAs. These protein-nucleic acid interactions are required for the integrity of the genome and the stability of the processes required by the central dogma. The integrity of genomic DNA requires the fidelity of the DNA polymerases that copy and repair the millions of DNA base pairs per cell. One polymerase that repair DNA in humans is DNA polymerase 棺. Studies of DNA polymerase 棺 (pol 棺) have shown that the interactions of pol 棺 with the target DNA are tightly regulated to maximize the fidelity of the enzyme. The wild type enzyme was monitored using NMR spectroscopy in order to study the effects of the four template nucleotides (adenosine, guanosine, thymine, and cytosine) have on the structure and dynamics of the enzyme. The wild type enzyme was shown to bind independently of the nucleotide |
| 일반주제명 | Chemistry. Biophysics. |
| 언어 | 영어 |
| 바로가기 | 
: 이 자료의 원문은 한국교육학술정보원에서 제공합니다. |
